大鼠胆总管结扎致肝硬化及肝肺综合征模型的建立

发布时间：2019-03-02 15:58

【摘要】：肝肺综合征(hepatopulmonary syndrome, HPS)是在慢性肝病和／或门脉高压的基础上出现肺内血管异常扩张,气体交换障碍,动脉血氧合作用异常。目前临床上缺乏明确的诊断标准,但在临床上较认可的标准有以下几点：1、慢性肝脏病变的基础；2、患者由卧位转为直立位血氧分压下降,在直立位时动脉氧分压降低约10%以上；3、CT薄层扫描发现肝肺综合症患者右下肺动脉支气管比例为2：1,而正常为1.2：1。大约有1/3-2/3的肝硬化患者可出现动脉氧分压下降,10-20%的病人出现肝肺综合征。90%的肝肺综合征患者,在早期取平卧时所测得的血气分析氧分压往往是正常的,但在直立位时动脉氧分压降低约10%以上,即发生所谓的直立性低氧血症。同时许多患者主诉从平卧到直立体位出现呼吸困难。平卧后可以缓解。国内外多数实验认为本病的发生机制与体内NO生成增加有关。本实验结扎雌性大鼠胆总管,使之发生胆汁淤积性肝硬化,通过肝病理检查证实大鼠肝硬化模型建立,对肝硬化大鼠取静脉血测NO-2/NO-3以及动脉血气分析证明肝肺综合征模型的成功建立及NO与肝肺综合征的关系,从而为后期的药物实验奠定基础,最终发现新的治疗肝肺综合征的药物。
[Abstract]:On the basis of chronic liver disease and / or portal hypertension, hepatopulmonary syndrome (hepatopulmonary syndrome, HPS) is characterized by abnormal dilation of pulmonary blood vessels, gas exchange disorder and abnormal arterial and blood oxygenation. At present, there is no definite diagnostic standard in clinic, but the more recognized criteria are as follows: (1) the basis of chronic liver disease; (2) the partial pressure of blood oxygen decreased from lying position to orthostatic position, and the partial pressure of arterial oxygen decreased by more than 10% in upright position; 3. The ratio of right inferior pulmonary artery bronchus to right inferior pulmonary artery bronchus in patients with hepatopulmonary syndrome was 2? 1, while that in normal patients was 1.2? There are about 1-3-2-3 cirrhosis patients with decreased arterial oxygen pressure, 10-20% of patients with hepatopulmonary syndrome, 90% of patients with hepatopulmonary syndrome, The oxygen partial pressure measured during early recumbent was usually normal, but the arterial oxygen partial pressure decreased by more than 10% at orthostatic position, that is, the so-called orthostatic hypoxemia occurred. At the same time, many patients complained of dyspnea from lying flat to upright position. It can be relieved after lying flat. Most experiments at home and abroad suggest that the pathogenesis of the disease is related to the increase of NO production in vivo. The common bile duct of female rats was ligated to cause cholestasis cirrhosis. The successful establishment of the model of hepatopulmonary syndrome and the relationship between NO and hepatopulmonary syndrome were proved by venous blood test of NO-2/NO-3 and arterial blood gas analysis in cirrhotic rats, thus laying the foundation for the later drug experiment. A new drug for the treatment of hepatopulmonary syndrome was eventually discovered.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332;R575;R563

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