耐锰微生物的筛
发布时间：2019-03-04 12:05
【摘要】：20世纪80年代以来兴起的微生物除锰技术,以其效果好、投资省等优点逐渐受到关注。微生物作为生态修复的一个主体,在重金属污染治理中起着重要作用。 本研究利用在湘潭鹤岭镇锰矿栾树恢复区采集的土壤样本,通过逐级提高Mn2+浓度的方法筛选和驯化获得优势耐锰菌株,采用优势菌株进行了除锰试验,分析测试了优势菌株的生长曲线、耐受性和相关生理生化特性,并对优势菌株进行了分类学鉴定。研究结果如下： (1)筛选获得了3株耐锰性强的杆菌(2、3、80号)。在低浓度范围内,Mn2+对筛选菌种的生长有促进作用,但在高Mn2+浓度点上,Mn2+抑制菌体生长。菌种抗Mn2+性能有一定差异,80号菌耐受性最强。 (2)在测试的最低Mn2+浓度(16mg/L)点上,3号菌种的锰的去除率最高(82.6%),其次是2号(72.3%),80号最低(64.1%),在测试的最高Mn2+浓度(约2000mg/L)点上,80号菌种的去除率最高(41.0%),其次是2号(22.0%),3号最低(18.3%),在Mn2+浓度约200mg/L点上,单一菌种和混合菌样本Mn2+的去除率最高(90%)。菌种混合后在每个浓度点上均保持较高的去除率。 (3)通过菌落形态观察、革兰氏染色、16s rDNA分子鉴定表明：菌株2为巨大芽孢杆菌；菌株3为产酸克雷伯氏杆菌；菌株80为弗氏柠檬酸杆菌。 (4)筛选的菌株在18～21h左右基本达到生长最高峰,稳定期较长；80号菌株生长比其他两株菌稍慢。3株菌的生长导致培养基pH值升高,这有利于Mn2+的氧化而形成氧化物沉淀。筛选的3株菌适应的pH值范围较宽(5-9)在温度28～37℃C之间,2、3、80号菌株最适生长温度分别为35℃、37℃、33℃。电镜扫描显示的特征说明,3株菌的除锰方式可能是将Mn2+氧化为MnO2沉淀。抗生素抗性试验表明,3号菌株能抗氨苄青霉素,在浓度达到200ug/ml时也能生长,但对硫酸卡那霉素及氯霉素敏感。2、80号菌对三种抗生素均敏感。 (5)从应用角度用泥炭土作为载体,制作了筛选菌株的菌制剂。
[Abstract]:Since 1980's, the technology of microbial manganese removal has been paid more and more attention due to its advantages of good effect, low investment and so on. As a main body of ecological remediation, microorganisms play an important role in the treatment of heavy metal pollution. In this study, soil samples collected from Luan tree restoration area of Heling Town Manganese Mine in Xiangtan were used to screen and acclimate the dominant manganese-tolerant strains by increasing the concentration of Mn2 step by step, and the manganese removal test was carried out by the dominant strains. The growth curve, tolerance, physiological and biochemical characteristics of the dominant strains were analyzed and tested, and the taxonomic identification of the dominant strains was carried out. The results were as follows: (1) three strains of manganese-tolerant bacilli (2,3,80) were obtained. In the low concentration range, Mn2 promoted the growth of the screened strain, but at the high Mn2 concentration point, Mn2 inhibited the growth of the strain. The strain had some difference in anti-Mn2 performance, and strain 80 had the strongest tolerance. (2) at the lowest Mn2 concentration (16mg/L) point tested, strain 3 had the highest manganese removal rate (82.6%), followed by No. 2 (72.3%), and No. 80 had the lowest removal rate (64.1%). At the highest Mn2 concentration (about 2000mg/L) point tested, strain 80 had the highest removal rate (41.0%), followed by No. 2 (22.0%), No. 3 (18.3%), and at the Mn2 concentration of about 200mg/L point, the removal rate of strain 80 was the highest (41.0%), followed by No. 2 (22.0%), the lowest (18.3%). The Mn2 removal rate of single strain and mixed bacteria sample was the highest (90%). After mixing, the bacteria kept high removal rate at each concentration point. (3) the colony morphology, Gram staining and 16s rDNA molecular identification showed that strain 2 was Bacillus giganticus, strain 3 was Klebsiella acidogenes, and strain 80 was citric acid bacillus. (4) the selected strains reached the highest growth peak at about 18 h after 21 h, and the stable period was longer; The growth of strain 80 was slightly slower than that of the other two strains. The growth of the three strains resulted in the increase of pH value of the medium, which was beneficial to the oxidation of Mn2 and the formation of oxide precipitation. The suitable pH ranges of the three strains were between 28 鈩，
本文编号：2434254
[Abstract]:Since 1980's, the technology of microbial manganese removal has been paid more and more attention due to its advantages of good effect, low investment and so on. As a main body of ecological remediation, microorganisms play an important role in the treatment of heavy metal pollution. In this study, soil samples collected from Luan tree restoration area of Heling Town Manganese Mine in Xiangtan were used to screen and acclimate the dominant manganese-tolerant strains by increasing the concentration of Mn2 step by step, and the manganese removal test was carried out by the dominant strains. The growth curve, tolerance, physiological and biochemical characteristics of the dominant strains were analyzed and tested, and the taxonomic identification of the dominant strains was carried out. The results were as follows: (1) three strains of manganese-tolerant bacilli (2,3,80) were obtained. In the low concentration range, Mn2 promoted the growth of the screened strain, but at the high Mn2 concentration point, Mn2 inhibited the growth of the strain. The strain had some difference in anti-Mn2 performance, and strain 80 had the strongest tolerance. (2) at the lowest Mn2 concentration (16mg/L) point tested, strain 3 had the highest manganese removal rate (82.6%), followed by No. 2 (72.3%), and No. 80 had the lowest removal rate (64.1%). At the highest Mn2 concentration (about 2000mg/L) point tested, strain 80 had the highest removal rate (41.0%), followed by No. 2 (22.0%), No. 3 (18.3%), and at the Mn2 concentration of about 200mg/L point, the removal rate of strain 80 was the highest (41.0%), followed by No. 2 (22.0%), the lowest (18.3%). The Mn2 removal rate of single strain and mixed bacteria sample was the highest (90%). After mixing, the bacteria kept high removal rate at each concentration point. (3) the colony morphology, Gram staining and 16s rDNA molecular identification showed that strain 2 was Bacillus giganticus, strain 3 was Klebsiella acidogenes, and strain 80 was citric acid bacillus. (4) the selected strains reached the highest growth peak at about 18 h after 21 h, and the stable period was longer; The growth of strain 80 was slightly slower than that of the other two strains. The growth of the three strains resulted in the increase of pH value of the medium, which was beneficial to the oxidation of Mn2 and the formation of oxide precipitation. The suitable pH ranges of the three strains were between 28 鈩，
本文编号：2434254
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