改良多囊卵巢综合征大鼠模型卵泡颗粒细胞中凋亡调控蛋白Bcl-2 Bax表达的研究

发布时间：2019-03-22 12:45

【摘要】：目的:探讨凋亡调控蛋白Bcl-2和Bax在改良多囊卵巢综合征(PCOS)大鼠模型卵巢的卵泡中颗粒细胞中的表达。方法:分别在来曲唑(Letrozole)和脱氢表雄酮(DHEA)造模基础上加用高脂乳剂,建立改良PCOS动物模型,结合阴道脱落细胞涂片、大鼠血清性激素水平、处死时体重增加幅度和卵巢形态学的改变进行模型验证,选用免疫组织化学法检测大鼠卵巢组织Bcl-2、Bax的表达。结果:模型建立成功。免疫组织化学结果显示高脂模型组、Letrozole模型组、Letrozole改良组、DHEA模型组和DHEA改良组Bax平均灰度均值均高于空白对照组,Bcl-2平均灰度均值均低于空白对照组,差异无统计学意义。结论:Bcl-2在卵巢的卵泡颗粒细胞中表达下调,Bax表达上调,推测两者表达改变可能是导致改良PCOS模型大鼠卵巢中颗粒细胞凋亡增多的关键因素。
[Abstract]:Aim: to investigate the expression of apoptosis regulatory proteins Bcl-2 and Bax in granulosa cells of ovarian follicles of modified polycystic ovary syndrome (PCOS) rats. Methods: on the basis of letrozole (Letrozole) and dehydroepiandrosterone (DHEA) models, a modified PCOS animal model was established by using high fat emulsion, combined with vaginal smears and serum sex hormone levels in rats. The weight gain and the morphological changes of the ovary were verified. The expression of Bcl-2,Bax in the ovary tissue of the rats was detected by immunohistochemical method. Results: the model was established successfully. The results of immunohistochemistry showed that the mean gray scale of Bax in high fat model group, Letrozole improved group, DHEA model group and DHEA improved group was higher than that in blank control group, while the average gray scale in Bcl-2 was lower than that in blank control group. The difference was not statistically significant. Conclusion: the expression of Bcl-2 is down-regulated and the expression of Bax is up-regulated in granulosa cells of ovary. It is suggested that the change of both expressions may be the key factors leading to the increase of apoptosis of granulosa cells in ovary of modified PCOS model rats.
【作者单位】：成都中医药大学;
【基金】：国家自然科学基金(编号:81470199) 成都中医药大学科技发展基金(编号:ZRMS201307)
【分类号】：R711.75;R-332

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