Gas7在大鼠斜角带核发育过程中的表达

发布时间：2019-03-24 12:32

【摘要】：目的研究大鼠发育过程中,生长休止蛋白7(growth arrest-specific protein 7,Gas7)在斜角带核(diagonal band nucleus,DB)的表达。方法应用RT-PCR方法、Western blot方法和免疫组织化学方法观察胚胎期16.5天(E16.5)、E20.5、出生当天(P0)、生后7天(P7)、P14、P21和2月龄(成年)大鼠DB的Gas7表达及定位。结果 RT-PCR和Western blot检测显示,在E16.5时Gas7表达最弱,其后逐渐增强,至P21时达到高峰;免疫组织化学染色显示,斜角带核水平支(horizontal limb of the diagonal band,HDB)在E16.5和E20.5时出现Gas7免疫反应阳性产物,Gas7阳性神经元出现于P0,至P21时阳性神经元数量最多,染色最深;斜角带核垂直支(vertical limb ofthe diagonal band,VDB)至P14时Gas7阳性神经元最多,P21和成年均有所减少。结论 Gas7的表达在大鼠DB发育过程中具有时间和空间上的特异性,提示Gas7可能参与大鼠DB的发育过程。
[Abstract]:Aim to study the expression of growth rest protein 7 (growth arrest-specific protein 7, Gas 7) in diagonal band nucleus (diagonal band nucleus,DB) during development in rats. Methods RT-PCR, Western blot method and immunohistochemical method were used to observe the embryonic stage of 16.5 days (E16.5), E20.5, the day of birth (P0), postnatal day 7 (P7), P14, Expression and localization of Gas7 in DB of P21 and 2 month old rats. Results RT-PCR and Western blot showed that the expression of Gas7 was the weakest at E16.5, then increased gradually and reached the peak at P21. Immunohistochemical staining showed that (horizontal limb of the diagonal band,HDB in the horizontal branch of the diagonal band showed Gas7 immunoreactive products at E16.5 and E20.5, Gas7 positive neurons appeared in P0, and the number of positive neurons at P21 was the most, and the number of positive neurons at E16.5 and E20.5 was more than that at E16.5 and E20.5, respectively. The staining was the deepest; From the vertical branch of the diagonal band nucleus (vertical limb ofthe diagonal band,VDB) to P14, the number of Gas7-positive neurons was the most, while the number of P21 and adult neurons was decreased. Conclusion the expression of Gas7 has temporal and spatial specificity during the development of rat DB, suggesting that Gas7 may be involved in the development of DB in rats.
【作者单位】：皖南医学院人体解剖学教研室;
【基金】：安徽省高校省级自然科学项目(11040606M162) 安徽省名师工作室(2014msgzs 152) 皖南医学院中青年科研基金项目(WK 201618)
【分类号】：R363

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