压力对肾功能影响的动物实验研究
发布时间：2019-03-24 14:50
【摘要】：目的通过模型加压兔子肾脏,监测加压前后肾功能变化,论证压力对肾功能的影响,根据实验结果给多囊肾临床治疗提供借鉴。方法将成年新西兰兔随机分为三组(A,B,C),A组兔子切除一侧肾脏,另一侧肾脏通过模型固定圆形气泡予肾脏加压;B组兔子切除一侧肾脏,另一侧肾脏通过模型固定塑料圆球予肾脏加压;C组兔子切除一侧肾脏,另一侧肾脏不予加压处理。分别监测实验组和对照组兔子术前术后肾功的变化(血肌酐、尿素)。对不同加压肾脏标本进行HE、masson和六氨银染色,了解肾脏加压后的病理改变。结果实验组A行加压术后肾功相对术前与对照组C相比无明显差异;实验组B行加压术后,肌酐、尿素升高明显,与术前、实验组A、对照组C比较均有明显差异(P0.01)。实验组C一侧肾切除后相比术前肾功能无明显差异。病理示:肾脏加压后,肾小球、肾小管、肾血管继发一系列损害肾功的病理改变。结论对肾脏施加的压力足够大的时候,压力致使肾脏发生相应病理改变,肌酐、尿素升高,肾功能明显下降。因此传统的囊肿去顶减压术,仍是有意义的对症治疗手段。
[Abstract]:Aim to investigate the effect of pressure on renal function by monitoring the changes of renal function before and after compression in rabbits, and to provide a reference for clinical treatment of polycystic kidney according to the experimental results. Methods Adult New Zealand rabbits were randomly divided into three groups (A, B, C), A). One side of the kidney was resected in group B, and the other was compressed by fixed plastic spheres in group C, and the other kidney was not treated with pressure in group C. The changes of renal function (serum creatinine and urea) were monitored before and after operation in the experimental group and the control group respectively. Renal specimens under different pressures were stained with HE,masson and silver hexa-ammonia to understand the pathological changes after renal compression. Results there was no significant difference in renal function between experimental group A and control group C before and after operation, but in experimental group B, creatinine and urea increased significantly after operation, and there was significant difference between group A and control group C before and after operation (P0.01). The renal function of experimental group A and control group C were significantly higher than those of control group (P0.01). There was no significant difference in renal function before and after C side nephrectomy in the experimental group. Pathology: after renal compression, glomeruli, tubules, and renal vessels were secondary to a series of pathological changes that damaged renal function. Conclusion when the pressure on the kidney is high enough, the renal pathological changes occur, the creatinine and urea increase, and the renal function decreases obviously. Therefore, the traditional decompression of cyst decapitation is still a meaningful symptomatic treatment.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332
本文编号：2446421
[Abstract]:Aim to investigate the effect of pressure on renal function by monitoring the changes of renal function before and after compression in rabbits, and to provide a reference for clinical treatment of polycystic kidney according to the experimental results. Methods Adult New Zealand rabbits were randomly divided into three groups (A, B, C), A). One side of the kidney was resected in group B, and the other was compressed by fixed plastic spheres in group C, and the other kidney was not treated with pressure in group C. The changes of renal function (serum creatinine and urea) were monitored before and after operation in the experimental group and the control group respectively. Renal specimens under different pressures were stained with HE,masson and silver hexa-ammonia to understand the pathological changes after renal compression. Results there was no significant difference in renal function between experimental group A and control group C before and after operation, but in experimental group B, creatinine and urea increased significantly after operation, and there was significant difference between group A and control group C before and after operation (P0.01). The renal function of experimental group A and control group C were significantly higher than those of control group (P0.01). There was no significant difference in renal function before and after C side nephrectomy in the experimental group. Pathology: after renal compression, glomeruli, tubules, and renal vessels were secondary to a series of pathological changes that damaged renal function. Conclusion when the pressure on the kidney is high enough, the renal pathological changes occur, the creatinine and urea increase, and the renal function decreases obviously. Therefore, the traditional decompression of cyst decapitation is still a meaningful symptomatic treatment.
【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332
【参考文献】
相关期刊论文 前1条
1 欧平;邓晓洪;郑军;王元中;;后腹腔镜肾囊肿去顶减压术治疗成人多囊肾[J];重庆医学;2009年15期
，本文编号：2446421
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