大肠癌肝转移动物模型建立方法的改进及评价

发布时间：2019-03-31 01:15

【摘要】：背景和目的：结直肠癌(CRC)是一种常见的严重威胁人类生命健康的恶性肿瘤。远处转移和浸润性生长是人类结直肠癌致死的主要原因,Cady等通过对罹患结肠癌死亡的患者进行尸检证实,其中约60%～70%的患者存在肝脏转移。因此,建立一种易于观察、生物学行为更为接近于临床表现的结直肠癌肝转移动物模型,对于了解结直肠癌生长、演进、转移的过程及正确评价各种治疗方案的疗效等都具有重要意义。目前现有的大肠癌肝转移模型建模周期较长,成功率较低,模型不稳定。我们针对这一缺点进行模型改良,从而建立高转移、高浸润、稳定的模型。方法：以近交系BALB/c小鼠为研究对象,随机分为两组,第一组选取鼠大肠癌细胞株CT26经皮下传3代后制成细胞悬液,将此悬液接种到小鼠脾脏被膜下,5min后切去脾脏。另一组将CT26细胞悬液直接接种于BALB/c小鼠脾脏内,5min后切除脾脏。观察两组小鼠成瘤情况、肝脏转移率、淋巴转移率、肺转移率及生存期,并对2种模型进行初步比较。留肝脏标本做病理检查。结果用spss17.0做统计学分析。结果：术后第12d实验组肝脏转移率(75%)高于对照组(28.6%)(P0.05)；术后第15d及21d后实验组肝脏转移率(93.7%、100%)也明显高于对照组(50%、66.7%)(P0.05),但肺脏转移率及淋巴转移率(12.5%、25%；26.7%、53.3%)与对照组(0、6.25%；0%、16.7%)相比无明显差异(PPP0.05)。平均生存期实验组为(23.56±3.17)d,对照组为(28.14土4.19)d。两组无明显统计学差别。病理结果证实肝转移肿瘤符合典型的低分化腺癌特征。结论：CT26细胞经小鼠皮下传代后建模比传统建模方法成瘤时间更短,转移率更高。
[Abstract]:Background and objective: colorectal cancer (CRC) is a common malignant tumor which is a serious threat to human life and health. Distant metastasis and invasive growth are the main causes of death in human colorectal cancer. Cady et al confirmed liver metastasis in about 60% of the patients who died of colon cancer by autopsy. Therefore, in order to understand the growth and progression of colorectal cancer, an animal model of liver metastasis of colorectal cancer is established, which is easy to observe and the biological behavior is more similar to the clinical manifestation. The process of metastasis and the correct evaluation of therapeutic effects are of great significance. At present, the current models of liver metastasis from colorectal cancer have a long period of modeling, a low success rate and unstable models. We improve the model to build a model with high metastasis, high infiltration and stability. Methods: inbred BALB/c mice were randomly divided into two groups. In the first group, a mouse colorectal cancer cell line CT26 was subcutaneously propagated for 3 generations to make a cell suspension. The suspension was inoculated under the membrane of the spleen of mice, and the spleen was cut off after 5min. In the other group, the CT26 cell suspension was inoculated directly into the spleen of BALB/c mice, and the spleen was resected after 5min. The tumorigenesis, liver metastasis rate, lymph node metastasis rate, lung metastasis rate and survival time of the two groups were observed. Liver specimens were left for pathological examination. Results spss17.0 was used for statistical analysis. Results: the liver metastasis rate in the experimental group (75%) was higher than that in the control group (28.6%) on the 12th day after operation (P0.05). The liver metastasis rate in the experimental group (93.7%, 100%) was significantly higher than that in the control group (50%, 66.7%) (P0.05), but the lung metastasis rate and lymph node metastasis rate (12.5%, 25%) were significantly higher than those in the control group (P0.05). There was no significant difference (PPP0.05) between 26.7%, 53.3% and the control group (0,6.25%, 16.7%). The mean survival time was (23.56 卤3.17) days in the experimental group and (28.14 卤4.19) d in the control group. There was no statistical difference between the two groups. The pathological results showed that the metastatic liver tumors were in accordance with the typical features of poorly differentiated adenocarcinoma. Conclusion: the time of tumorigenesis and metastasis rate of CT26 cells after subcutaneously passage is shorter and higher than that of traditional modeling methods.
【学位授予单位】：新疆医科大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R-332

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