鼠衣原体在不同遗传背景小鼠中病变差异性与炎症细胞动态变化

发布时间：2019-04-01 22:58

【摘要】：【目的】初步分析鼠衣原体(Chlamydia muridarum,Cm)致不同遗传背景小鼠生殖道病变的原因,探讨炎症细胞动态变化在衣原体感染致不同人群病变差异的可能机制。【方法】用2×105 Inclusion forming units(IFUs)/小鼠的Cm剂量经子宫角(Intrauterine inoculation,iu)途径分别感染DBA/2J鼠和A/J鼠;在感染60 d处死两组小鼠,肉眼观察其输卵管水肿程度,显微镜下观察评估输卵管的扩张程度及炎症反应程度;每组小鼠在感染后3、7、14、21、28、35、42、49、56、60 d分别取阴道分泌物并检测其中衣原体包涵体的数量;在第14天每组各处死5只小鼠,检测上生殖道(输卵管及卵巢)组织匀浆中衣原体包涵体数量及炎症因子水平;同时,在3、28、35 d每组分别处死5只小鼠,显微镜下鉴定病变组织炎症细胞种类及变化。【结果】两组小鼠在感染60 d肉眼观察下的生殖道病变率有明显统计学差异,且两组小鼠病变组织的输卵管扩张程度也具有统计学差异,但炎症反应程度评分无统计学差异;DBA/2J与A/J两组小鼠在阴道分泌物的衣原体包涵体检测及小鼠阳性检出率中并无显著性差异;感染后14 d两组小鼠在上生殖道组织匀浆中的包涵体检出量无统计学差异,但某些炎症因子水平具有统计学差异;感染早期两组小鼠病变组织中嗜中性粒细胞浸润增加,但无明显差异,第28天DBA/2J小鼠病变组织中出现较多嗜酸性粒细胞,并在第35天与A/J小鼠形成统计学差异。【结论】DBA/2J与A/J小鼠在以iu方式感染等量Cm后,引起小鼠的生殖道病变差异可能与嗜酸性粒细胞参与的炎症反应有关。
[Abstract]:[objective] to analyze the causes of reproductive tract lesions caused by Chlamydia muridarum,Cm in mice with different genetic background. To explore the possible mechanism of the dynamic changes of inflammatory cells in different groups caused by chlamydia infection. [methods] DBA/ 2J mice and A / J mice were infected with 2 脳 10 5 Inclusion forming units (IFUs) / mouse Cm dose through the uterine horn (Intrauterine inoculation,iu) pathway, respectively. The mice of two groups were killed at 60 days after infection, and the degree of tubal edema was observed by naked eye, and the degree of tubal dilation and inflammatory reaction were observed and evaluated under microscope. At 3, 7, 14, 21, 28, 35, 42, 49, 56 days after infection, vaginal secretions of each group were taken and the number of the inclusion bodies of the chlamydia was detected respectively in the mice of each group at 3,7,14,21,28,35,42,49,56 days after infection. On the 14th day, 5 mice in each group were killed to detect the number of chlamydia inclusion bodies and the levels of inflammatory factors in the homogenate of the upper genital tract (oviduct and ovary). At the same time, 5 mice in each group were killed at 3,28,35 days to identify the types and changes of inflammatory cells in pathological tissue under microscope. [results] there was significant difference in reproductive tract pathological rate under naked eye observation on 60 days after infection between the two groups. The degree of tubal dilatation was also statistically different between the two groups of mice, but there was no statistical difference in the degree of inflammatory reaction. There was no significant difference between the two groups in the detection of coat body inclusion body in vaginal secretion and the positive detection rate of mice in the two groups of DBA/2J and AqJ. The results showed that there was no significant difference between the two groups. On the 14th day after infection, there was no statistical difference in the amount of physical examination in the homogenate of the upper genital tract between the two groups, but the levels of some inflammatory factors had statistical difference. In the early stage of infection, neutrophil infiltration increased, but there was no significant difference between the two groups. On the 28th day, there were more eosinophils in the pathological tissues of DBA/2J mice. [conclusion] the difference of reproductive tract pathological changes between DBA/2J and A / J mice may be related to the inflammatory reaction of eosinophils after the same amount of Cm was infected in the way of iu in mice on the 35th day. [conclusion] the difference of reproductive tract pathological changes in mice may be related to the inflammatory reaction involved in eosinophils.
【作者单位】：南华大学医学院病原生物学研究所;中南大学湘雅二医院妇产科;中南大学湘雅二医院病理科;
【基金】：国家自然科学基金项目(No.31570179,30901352) 湖南省自然科学基金项目(No.13JJ4072) 湖南省教育厅优秀青年基金项目(No.12B109) 湖南省分子靶标新药研究协同创新中心资助项目(No.2015-351) 特殊病原体防控湖南省重点实验室资助项目(No.2014-5) 湖南省重点学科资助项目(No.2011-76)~~
【分类号】：R374

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