公路运输对新西兰兔血液及生化指标的影响

发布时间：2019-04-19 00:17

【摘要】：目的观察公路运输对新西兰兔血液常规和生化指标的影响。方法选用12只健康普通级新西兰兔进行2 h公路运输,分别在运输前,运输后0、24、48、72、96 h采集血液获得检测样本。通过血液分析仪检测血常规指标:白细胞(WBC)、红细胞(RBC)、血红蛋白量(HGB)、红细胞压积(MCV)、平均红细胞血红蛋白含量(MCH)、平均红细胞血红蛋白浓度(MCHC)、血小板(PLT)。通过全自动生化分析仪检测:肝功能指标:丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、白蛋白(ALB)、总蛋白(TP);肾功能指标:尿素氮(UREA)、肌酐(CREA)、尿酸(UA);脂代谢指标:甘油三酯(TG)、总胆固醇(COHL);糖代谢指标:葡萄糖(GLU);炎性指标:超敏感C-反应蛋白(CRP);血清酶:α-淀粉酶(AMYL)、肌酸激酶(CK)。结果与运输前比较,运输结束后:血常规指标中,WBC先升高(P0.05或P0.01)后降低,RBC、HGB、HCT、PLT均先降低(P0.05或P0.01)后升高,MCV在运输结束96 h时显著高于运输前(P0.05);肝功能指标中,ALT、AST先升高(P0.05或P0.01)后降低,TP、ALB先降低(P0.05或P0.01)后升高;肾功能指标中,BUN先升高(P0.05或P0.01)后降低,CREA先降低(P0.01)后升高;脂代谢指标中,TG先降低(P0.01)后升高;糖代谢指标GLU在运输结束24 h时显著低于运输前(P0.01);除MCV外,各项指标均在运输结束96 h时与运输前无明显差异。结论公路运输会引起新西兰兔血液常规、肝肾功能指标、脂代谢指标、糖代谢指标及肌酸激酶指标的变化,且各指标在96 h内基本恢复至运输前水平。
[Abstract]:Objective to observe the effect of road transportation on blood routine and biochemical indexes in New Zealand rabbits. Methods 12 normal New Zealand rabbits were used for 2 hours road transportation. Blood samples were collected before and 0,24,48,72,96 hours before and after transportation. Blood routine indexes were measured by blood analyzer: leukocyte (WBC), red blood cell (RBC), hemoglobin quantity (HGB), hematocrit (MCV), mean red blood cell hemoglobin content (MCH), mean red blood cell hemoglobin concentration (MCHC), Platelet (PLT). Liver function index: alanine aminotransferase (ALT), aspartate aminotransferase (AST), albumin (ALB), total protein (TP); Renal function index: urea nitrogen, (UREA), creatinine, (CREA), uric acid, (UA); lipid metabolism, triglyceride (TG), total cholesterol, (COHL);, glucose metabolism, glucose (GLU); Inflammatory index: hypersensitive C-reactive protein (CRP); serum enzyme: 伪-amylase (AMYL), creatine kinase (CK). Results compared with pre-transportation, WBC increased first (P0.05 or P0.01) and then decreased, and RBC,HGB,HCT,PLT decreased first (P0.05 or P0.01) and then increased in blood routine indexes at the end of transportation. At the end of 96 h, MCV was significantly higher than that before transport (P0.05). Among the indexes of liver function, ALT,AST increased first (P0.05 or P0.01) and then decreased, while TP,ALB decreased first (P0.05 or P0.01) and then increased; Among the indexes of renal function, BUN increased first (P0.05 or P0.01) and then decreased, CREA decreased first (P0.01) and then increased; among lipid metabolism indexes, TG first decreased (P0.01) and then increased. The glucose metabolism index (GLU) was significantly lower at 24 h after transport than that before transport (P0.01), except for MCV, there was no significant difference between before and 96 h after transport (P < 0.01). Conclusion Road transportation can cause changes in blood routine, liver and kidney function, lipid metabolism, glucose metabolism and creatine kinase in New Zealand rabbits, and all indexes return to pre-transport level within 96 hours.
【作者单位】：广州中医药大学;梅州市第二中医医院针灸科;广州中医药大学实验动物中心;
【基金】：广东省科技厅科技计划项目(2015A030302073);广东省科技厅科技计划项目(2013B040200033)
【分类号】：R-33

【参考文献】