斑马鱼脑出血模型的建立及丹参在该模型上改善脑出血的研究

发布时间：2019-04-23 16:06

【摘要】：目的:建立阿托伐他汀诱导的斑马鱼脑出血模型,评价丹参煎液及其水溶性单体成分对斑马鱼脑出血的影响。方法:选用转基因斑马鱼Tg(fli-1:EGFP)and Tg(gata1:DsRed)胚胎为研究载体,用阿托伐他汀诱导斑马鱼脑出血,建立脑出血斑马鱼模型,干预不同浓度的丹参煎液、丹酚酸A、丹酚酸B、丹参素,在荧光显微镜下拍照观察,计算斑马鱼脑出血面积和出血率。结果:丹参煎液、丹酚酸A、丹酚酸B、丹参素均可减少斑马鱼脑出血面积,丹参煎液的作用最为明显。而丹酚酸B不仅能减少出血面积,同时能够降低出血率。结论:丹参其煎液和部分水溶性成分丹酚酸A、丹酚酸B、丹参素均对斑马鱼脑出血有改善作用。其中丹酚酸B改善斑马鱼脑出血的作用较为明显,值得进一步研究。
[Abstract]:Aim: to establish a model of zebra fish intracerebral hemorrhage (ICH) induced by Atto vastatin and evaluate the effect of Salvia miltiorrhiza decoction and its water soluble monomers on ICH of zebrafish. Methods: transgenic zebrafish Tg (fli-1:EGFP) and Tg (gata1:DsRed) embryos were used to induce intracerebral hemorrhage (ICH) of zebra fish induced by Atto vastatin. The model of intracerebral hemorrhage was established to interfere with different concentrations of Salvia miltiorrhiza decoction and Salvianolic acid (SA). Salvianolic acid B and Danshensu were photographed and observed under fluorescence microscope to calculate the cerebral hemorrhage area and bleeding rate of zebra fish. Results: salvia miltiorrhiza decoction, Salvianolic acid A, Salvianolic acid B and Danshensu could reduce the area of cerebral hemorrhage of zebra fish, and the effect of Salvia miltiorrhiza decoction was the most obvious. Salvianolic acid B can not only reduce the bleeding area, but also reduce the bleeding rate. Conclusion: Salvianolic acid A, Salvianolic acid B and Danshensu can improve cerebral hemorrhage of zebra fish. The effect of Salvianolic acid B on improving cerebral hemorrhage of zebrafish is obvious, which is worthy of further study.
【作者单位】：上海中医药大学附属龙华医院;
【基金】：国家自然科学基金青年科学基金项目(81603549) 上海市加强公共卫生体系建设三年行动计划项目(GWIV-28) 上海市科委基金项目(13ZR1442400,16ZR1437600,14401972501) 上海市卫计委科研基金项目(20154Y0052) 上海中医药大学预算内项目(2014YSN31)
【分类号】：R285.5;R-332

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