波动性高糖对巨噬细胞中脂肪细胞脂肪酸结合蛋白表达的影响
发布时间：2019-04-26 22:45
【摘要】：目的探讨波动性高糖在THP-1巨噬细胞中通过Toll样受体4/c-Jun氨基末端激酶(TLR4/JNK)信号转导通路对脂肪细胞脂肪酸结合蛋白(A-FABP)表达的影响。 方法将佛波酯(PMA)加入培养的THP-1人单核细胞株中,诱导细胞分化为THP-1巨噬细胞,分为以下几组进行干预：细胞培养液组,甘露醇组(20mmol/),恒定低糖组(5mmol/1)、恒定高糖组(20mmol/1)、脂多糖组(100ng/ml)、波动性高糖1组(5mmol/l或20mmol/l,每12小时换液一次)、波动性高糖2组(5mmol/l或20mmol/l,每8小时换液一次)。以上各组又分别加入磷酸化JNK抑制剂SP600125或空白载体,干预72小时后测定THP-1巨噬细胞上TLR4、细胞内磷酸化JNK和A-FABP的表达水平,以及细胞上清液中IL-1β和TNF-α的表达水平。 结果恒定高糖组TLR4、细胞内磷酸化JNK和A-FABP、TNF-α和IL-1β水平均较恒定低糖组升高。波动性高糖组较恒定性高糖组更高,且波动性高糖2组高于波动性高糖1组(P均0.05)。加入磷酸化JNK抑制剂SP600125后,与加入空白载体组相比,THP-1巨噬细胞表达的TLR4蛋白以及mRNA水平变化差别没有统计学意义(P均0.05),而磷酸化JNK、A-FABP蛋白和mRNA表达水平以及细胞上清液中TNF-α和IL-1β水平均降低,差异有统计学意义(P均0.05)。 结论高糖可以通过诱导THP-1巨噬细胞上TLR4/磷酸JNK信号通路的活化而促进A-FABP的表达以及炎症因子的释放；与恒定性高糖相比,波动性高糖能进一步诱导THP-1巨噬细胞上TLR4／磷酸化JNK信号通路活化、A-FABP的表达以及炎症因子的分泌。
[Abstract]:Aim to investigate the effect of volatile high glucose on the expression of fatty acid binding protein (A-FABP) in adipocytes through Toll-like receptor 4/c-Jun N-terminal kinase (TLR4/JNK) signal transduction pathway in THP-1 macrophages. Methods the cells were induced to differentiate into THP-1 macrophages by adding phorbol ester (PMA) into the cultured human monocytes of THP-1 and were divided into the following groups: cell culture solution group, mannitol group (20mmol/), and control group. Constant low glucose group (5mmol/1), constant high glucose group (20mmol/1), lipopolysaccharide group (100ng/ml), fluctuating high glucose group 1 (5mmol/l or 20 mmol / ml, every 12 hours), fluctuating high glucose group 2 (5mmol/l or 20 mmol / ml), Change fluid every 8 hours) The phosphorylated JNK inhibitor SP600125 or blank vector were added to the above groups. 72 hours after intervention, the expression levels of phosphorylated JNK and A-FABP in TLR4, cells were measured. And the expression of IL-1 尾 and TNF- 伪 in the supernatant. Results the levels of phosphorylated JNK and 伪-IL-1-伪 and IL-1-尾 in TLR4, cells were significantly higher than those in low glucose group (P < 0.05). The fluctuating high glucose group was higher than the constant high glucose group, and the fluctuating high glucose group 2 was higher than the fluctuating high glucose group 1 (all P 0.05). When phosphorylated JNK inhibitor SP600125 was added, there was no significant difference in the expression of TLR4 protein and mRNA in THP-1 macrophages compared with the blank vector group (P0.05), but the phosphorylated JNK, was not significantly different from that in the blank vector group (P0.05). The expression levels of A-FABP protein and mRNA and the levels of TNF- 伪 and IL-1 尾 in the supernatant were significantly lower than those in the control group (P0.05). Conclusion High glucose can promote the expression of A-FABP and the release of inflammatory factors by inducing the activation of TLR4/ phosphate JNK signaling pathway in THP-1 macrophages. Compared with the constant high glucose, volatile high glucose could further induce the activation of TLR4/ phosphorylated JNK signaling pathway, the expression of A-FABP and the secretion of inflammatory factors in THP-1 macrophages.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
本文编号：2466457
[Abstract]:Aim to investigate the effect of volatile high glucose on the expression of fatty acid binding protein (A-FABP) in adipocytes through Toll-like receptor 4/c-Jun N-terminal kinase (TLR4/JNK) signal transduction pathway in THP-1 macrophages. Methods the cells were induced to differentiate into THP-1 macrophages by adding phorbol ester (PMA) into the cultured human monocytes of THP-1 and were divided into the following groups: cell culture solution group, mannitol group (20mmol/), and control group. Constant low glucose group (5mmol/1), constant high glucose group (20mmol/1), lipopolysaccharide group (100ng/ml), fluctuating high glucose group 1 (5mmol/l or 20 mmol / ml, every 12 hours), fluctuating high glucose group 2 (5mmol/l or 20 mmol / ml), Change fluid every 8 hours) The phosphorylated JNK inhibitor SP600125 or blank vector were added to the above groups. 72 hours after intervention, the expression levels of phosphorylated JNK and A-FABP in TLR4, cells were measured. And the expression of IL-1 尾 and TNF- 伪 in the supernatant. Results the levels of phosphorylated JNK and 伪-IL-1-伪 and IL-1-尾 in TLR4, cells were significantly higher than those in low glucose group (P < 0.05). The fluctuating high glucose group was higher than the constant high glucose group, and the fluctuating high glucose group 2 was higher than the fluctuating high glucose group 1 (all P 0.05). When phosphorylated JNK inhibitor SP600125 was added, there was no significant difference in the expression of TLR4 protein and mRNA in THP-1 macrophages compared with the blank vector group (P0.05), but the phosphorylated JNK, was not significantly different from that in the blank vector group (P0.05). The expression levels of A-FABP protein and mRNA and the levels of TNF- 伪 and IL-1 尾 in the supernatant were significantly lower than those in the control group (P0.05). Conclusion High glucose can promote the expression of A-FABP and the release of inflammatory factors by inducing the activation of TLR4/ phosphate JNK signaling pathway in THP-1 macrophages. Compared with the constant high glucose, volatile high glucose could further induce the activation of TLR4/ phosphorylated JNK signaling pathway, the expression of A-FABP and the secretion of inflammatory factors in THP-1 macrophages.
【学位授予单位】：中南大学
【学位级别】：硕士
【学位授予年份】：2012
【分类号】：R363
【参考文献】
相关期刊论文 前1条
1 都健 ,曾芙蓉 ,赵玉岩 ,刘国良;胰岛素抵抗大鼠血管内皮细胞的形态学变化[J];中华老年医学杂志;2005年07期
，本文编号：2466457
本文链接：https://www.wllwen.com/xiyixuelunwen/2466457.html
最近更新
教材专著
