当归挥发油对高血脂模型大鼠的降血脂作用及血管内皮保护作用

发布时间：2019-04-30 21:06

【摘要】：目的观察当归挥发油对高血脂模型大鼠的降血脂作用及血管内皮保护作用。方法 SD大鼠72只,随机分为正常对照组12只和造模组60只,造模组大鼠高脂饲料喂养并灌胃丙基硫氧嘧啶溶液(50 mg/kg),连续4周,造模成功后随机分为模型对照组、氟伐他汀组(0.5 mg/kg)、当归挥发油(ASVO)高、中、低剂量组(60、30、15mg/kg),每组各12只,连续灌胃给药3周,末次给药60 min后股动脉取血,分离血清,测定总胆固醇(TC)、甘油三酯(TG)、高密度脂蛋白胆固醇(HDLC)、低密度脂蛋白胆固醇(LDLC)含量,计算动脉粥样硬化指数(AI);分离胸主动脉,10%甲醛固定,石蜡包埋,切片,HE染色观察大鼠胸主动脉内皮形态改变,ELISA法测定大鼠血浆内皮素1(ET-1)、血清一氧化氮(NO)和血浆血管性假血友病因子(v WF)的含量。结果模型大鼠血清TC、LDLC水平及AI显著升高(P0.05或P0.01),当归挥发油高、中剂量能够降低高血脂大鼠血清TC、LDLC水平及AI(P0.05);模型大鼠血浆ET-1和血清v WF水平显著升高、血清NO水平显著下降(P0.01),当归挥发油高、中剂量均能降低血浆ET-1和血清v WF水平(P0.05),升高血清NO水平(P0.01)。光镜下可见模型大鼠胸主动脉内皮结构改变明显,而当归挥发油高、中剂量组病变减轻。结论当归挥发油对高血脂模型大鼠有一定的降脂作用,并能有效改善血管内皮结构的损伤。
[Abstract]:Objective to observe the effect of essential oil of Angelica sinensis on blood lipid lowering and vascular endothelial protection in hyperlipidemia model rats. Methods 72 SD rats were randomly divided into the control group (n = 12) and the model group (n = 60). The rats in the model group were fed with high fat diet and given intragastric administration of propylthiouracil solution (50 mg/kg) for 4 weeks. The rats in the model group were randomly divided into model control group (n = 12) and model control group (n = 60). Fluvastatin group (0.5 mg/kg), Angelica volatile oil (ASVO) high, middle and low dose group (60,30,15 mg / kg), 12 rats in each group were given intragastrically for 3 weeks, 60 min after the last administration, blood was collected from femoral artery and serum was separated. Determination of total cholesterol, (TC), triglyceride, (TG), high density lipoprotein cholesterol, (HDLC), low density lipoprotein cholesterol (LDLC), and calculation of atherosclerosis index (AI); The thoracic aorta was isolated, fixed with 10% formaldehyde, embedded in paraffin, sectioned and stained with HE. Plasma endothelin-1 (ET-1) was measured by ELISA method. Serum nitric oxide (NO) and plasma von Willebrand factor (v WF). Results the levels of serum TC,LDLC and AI were significantly increased in model rats (P0.05 or P0.01). The levels of serum TC,LDLC and AI in hyperlipidemic rats were significantly decreased by middle dose of Angelica sinensis (P0.05). The levels of plasma ET-1 and serum v-WF in model rats were significantly increased, while serum NO levels were significantly decreased (P0.01). The levels of plasma ET-1 and serum v-WF were decreased in middle dose and high dose of Angelica sinensis volatile oil (P0.05). The level of serum NO was increased (P0.01). There were obvious changes in the endothelium structure of thoracic aorta in the model rats under light microscope, while the high volatile oil of Angelica sinensis reduced the pathological changes in the middle-dose group. Conclusion the essential oil of Angelica sinensis can reduce lipid in hyperlipidemia model rats, and can effectively improve the damage of vascular endothelial structure.
【作者单位】：甘肃中医药大学;甘肃省中药药理与毒理学重点实验室;甘肃省高校中(藏)药化学与质量研究省级重点实验室;
【基金】：甘肃省自然科学基金项目(1208RJZA215) 甘肃省中药药理与毒理学重点实验室开放基金项目(ZDSYS-KJ-2012-004)
【分类号】：R285.5;R-332

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