枸杞多糖对精神分裂症模型大鼠海马中GluR1表达及认知功能的影响

发布时间：2019-05-05 06:22

【摘要】：目的探讨枸杞多糖(LBP)对精神分裂症模型(Sz)大鼠海马中Glu R1表达及认知功能影响。方法选取36只健康SD大鼠,随机分3组,每组12只,分别为正常对照组、模型组及药物干预组(Sz+LBP)。Sz及Sz+LBP腹腔注射地卓西平马来酸盐0.6 mg/(kg·d),连续注射14 d制造精神分裂症模型,Sz+LBP同时灌胃枸杞多糖100 mg/(kg·d)。正常对照组在相应时段注射等剂量的正常生理盐水。造模成功后,分别评估各组大鼠的行为学改变,进行Morris水迷宫,比较3组大鼠的定位航行和空间探索时间以及采用免疫荧光染色、Western blot法测定海马中GluR1蛋白的表达。结果 Sz的运动量、共济失调、刻板行为评分以及海马中GluR1均高于正常对照组(P0.01),Sz+LBP的运动量、共济失调及刻板行为评分均低于Sz,明显抑制精神分裂症大鼠海马中GluR1的表达(P0.01)。结论枸杞多糖对精神分裂症模型大鼠海马具有保护作用,其机制与海马中GluR1表达来改善其认知功能相关。
[Abstract]:Objective to investigate the effect of Lycium barbarum polysaccharide (LBP) on Glu R1 expression and cognitive function in hippocampus of schizophrenia model (Sz) rats. Methods 36 healthy SD rats were randomly divided into 3 groups (n = 12 in each group): normal control group, model group and drug intervention group. (Sz LBP) .z and Sz LBP were injected intraperitoneally with 6. 6 mg/ (kg 路d),) of diazepine. The model of schizophrenia was made by continuous injection for 14 days., Sz LBP was injected with 100 mg/ (kg 路d).) of Lycium barbarum polysaccharide at the same time. The normal control group was injected with the same dose of normal saline at the corresponding time. After successful modeling, the behavioral changes of each group were evaluated and the Morris water maze was carried out. The time of localization and space exploration were compared among the three groups, and the expression of GluR1 protein in hippocampus was detected by immunofluorescence staining, Western blot method. Results the amount of exercise, ataxia, stereotype behavior score and GluR1 in hippocampus of Sz were higher than those of normal control group (P0.01), Sz LBP, ataxia and stereotype behavior score were lower than that of Sz,). The expression of GluR1 in hippocampus of schizophrenic rats was significantly inhibited (P0.01). Conclusion Lycium barbarum polysaccharide has protective effect on hippocampus of schizophrenia model rats, and its mechanism is related to the expression of GluR1 in hippocampus to improve its cognitive function.
【作者单位】：广州医科大学附属脑科医院(广州市惠爱医院)精神科;南方医科大学中医药学院;
【分类号】：R285.5;R-332

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