胫骨内注射大鼠骨转移疼痛模型的显像研究

发布时间：2019-05-14 22:33

【摘要】：目的:探讨胫骨内注射法建立的大鼠乳腺癌骨转移疼痛模型骨病灶的影像学确认方法。方法:将Walker 256大鼠乳腺癌腹水瘤注射入Wistar大鼠胫骨腔内,制作乳腺癌骨癌痛模型;以胫骨内注射等量PBS溶液作为对照组。使用von-Frey法,测量大鼠后足机械痛觉阈值,观察各组疼痛变化。对模型组和对照组进行~(99mTc)-MDP骨显像和CT成像,观察骨病灶的骨代谢变化及骨质结构的变化。结果:造模的第3~5天,乳腺癌骨转移疼痛大鼠的机械痛觉阈值较基础值明显下降(P0.01),并持续下降83.2%左右,至第9天左右达到最痛(P0.001),后下降曲线趋于缓慢变化。造模3~5天后,模型组机械痛觉阈值显著低于对照组(P0.01),这个差别持续增大至第9天左右。~(99mTc)-MDP骨显像显示所有模型组大鼠胫骨种植肿瘤细胞处放射性摄取显著高于对侧,CT见造模侧胫骨上段骨质破坏,对侧骨质结构正常,未见其他部位骨病灶存在。而对照组所有大鼠骨显像示两侧胫骨放射性分布对称,CT显示两侧骨质结构正常。结论:胫骨内注射法大鼠骨癌痛模型疼痛确切。~(99mTc)-MDP骨显像以及CT可以从骨代谢和骨结构评估骨癌痛模型病灶的发生,是确认此类骨转移疼痛模型造模的成功较好的影像学方法。
[Abstract]:Objective: to investigate the imaging confirmation of bone lesions in rat breast cancer bone metastasis pain model established by intra-tibia injection. Methods: Walker 256 rats were injected into the tibia cavity of Wistar rats to establish the model of breast cancer pain, and the same amount of PBS solution was injected into the tibia as the control group. The mechanical pain threshold of posterior foot of rats was measured by von-Frey method, and the changes of pain in each group were observed. ~ (99mTc)-MDP bone imaging and CT imaging were performed in the model group and the control group to observe the changes of bone metabolism and bone structure. Results: on the 3rd to 5th day, the mechanical pain threshold of rats with bone metastasis pain of breast cancer was significantly lower than that of the basic value (P0.01), and continued to decrease by 83.2%, reaching the highest pain on the 9th day (P0.001). The post-decline curve tends to change slowly. After 5 days of modeling, the threshold of mechanical pain in the model group was significantly lower than that in the control group (P 0.01). The difference continued to increase until the 9th day. ~ (99mTc)-MDP bone imaging showed that the radioactivity uptake of tumor cells in the tibia of all model groups was significantly higher than that of the contralateral side. CT showed bone destruction in the upper tibia of the model side, and the contralateral bone structure was normal. No bone lesions were found in other parts. In the control group, the radioactivity distribution of both tibia was symmetrical on bone imaging, and the bone structure of both sides was normal on CT. Conclusion: the pain of bone cancer pain model in rats by intra-tibia injection is accurate. ~ (99mTc)-MDP bone imaging and CT can evaluate the occurrence of bone cancer pain model from bone metabolism and bone structure. It is a good imaging method to confirm the success of this kind of bone metastasis pain model.
【作者单位】：复旦大学附属华山医院核医学科;同济大学附属东方医院核医学科;复旦大学上海医学院中西医结合系;
【基金】：国家自然科学基金No.81201113 上海市卫计委青年科学基金项目(20124Y069)~~
【分类号】：R-332;R737.9;R738.1

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