采用唾液淀粉酶活性指标对脾虚模型大鼠唾液采集方法的评价研究
发布时间:2019-05-15 01:17
【摘要】:目的:评价脾虚模型大鼠唾液采集方法的可行性。方法:采集利血平所致脾虚组大鼠和正常组大鼠各24例酸刺激前后的唾液,其中刺激后的唾液以0.4 mol/l 0.50 cm×0.50 cm柠檬酸滤纸,每隔2.5 min刺激大鼠舌尖30 s,获得刺激后0~5 min、6~10 min和11~15 min的唾液。Bernfeld法测定唾液淀粉酶(s AA)活性,计算s AA活性比值。结果:脾虚组衰竭明显,体质量比正常组显著降低;正常组与分组前所有大鼠刺激前和刺激后s AA活性及其活性比值比较差异无统计学意义;脾虚组酸刺激前的s AA活性与正常组比较差异无统计学意义,酸刺激后3个时间段的s AA活性和活性比值均比正常组显著降低。结论:建立唾液采集方法经s AA活性分析,表现出与人较为一致的趋势,可以用于脾虚大鼠s AA相关研究。
[Abstract]:Objective: to evaluate the feasibility of saliva collection in rats with spleen deficiency. Methods: saliva was collected from 24 rats in spleen deficiency group and 24 rats in normal group before and after acid stimulation induced by reserpine. The saliva after stimulation was stimulated with 0.4 mol/l 0.50 cm 脳 0.50 cm citric acid filter paper every 2.5 min. Saliva of 0 鈮,
本文编号:2477201
[Abstract]:Objective: to evaluate the feasibility of saliva collection in rats with spleen deficiency. Methods: saliva was collected from 24 rats in spleen deficiency group and 24 rats in normal group before and after acid stimulation induced by reserpine. The saliva after stimulation was stimulated with 0.4 mol/l 0.50 cm 脳 0.50 cm citric acid filter paper every 2.5 min. Saliva of 0 鈮,
本文编号:2477201
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