新蛋白C7orf42促进大鼠肝细胞BRL-3A增殖

发布时间：2019-05-17 13:48

【摘要】：目的探讨未知功能蛋白C7orf42对体外培养的大鼠肝细胞BRL-3A增殖的影响。方法基因干涉下调C7orf42表达后,用MTT、Ed U掺入法检测C7orf42对BRL-3A细胞增殖的影响,流式细胞术检测细胞周期进程以及Reat-time PCR和Western blotting检测细胞增殖相关基因表达。结果 MTT法检测表明,转染C7orf42干涉片段后48h,干涉组比阴性对照组细胞活力降低11%(P0.05);Ed U掺入法检测表明,实验组的Ed U阳性细胞比率比对照组降低了13%(P0.05);流式细胞术检测表明,干涉组比阴性对照组S+G2/M期的细胞数降低12%(P0.05);Reat-time PCR检测表明,干涉C7orf42表达后细胞增殖相关基因JUN、CCND1、MYC和CCNA2的表达分别下调26%、31%、37%和14%(P0.05);Western blotting检测表明,干涉C7orf42表达后细胞增殖相关蛋白JUN、CCND1、MYC和CCNA2的表达分别下调59%、54%、18%和27%(P0.05)。结论 C7orf42可能通过上调细胞增殖相关蛋白JUN、CCND1、MYC和CCNA2的表达,促进体外培养的大鼠肝细胞BRL-3A增殖。
[Abstract]:Objective to investigate the effect of unknown functional protein C7orf42 on the proliferation of rat hepatocytes BRL-3A in vitro. Methods after down-regulating the expression of C7orf42 by gene interference, the effect of C7orf42 on the proliferation of BRL-3A cells was detected by MTT,Ed U incorporation, the cell cycle process was detected by flow cytometry, and the expression of genes related to cell proliferation was detected by Reat-time PCR and Western blotting. Results MTT assay showed that 48 hours after C7orf42 interference, the cell vitality of the interference group was 11% lower than that of the negative control group (P 0.05). The results of Ed U incorporation showed that the percentage of Ed U positive cells in the experimental group was 13% lower than that in the control group (P 0.05), and the number of cells in S G 2 鈮，

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