Smad蛋白抑制剂SIS3对细粒棘球蚴原头节的作用研究
发布时间：2019-05-18 02:52
【摘要】：目的研究Smad蛋白抑制剂SIS3对体外细粒棘球蚴原头节的影响。方法建立体外细粒棘球蚴培养体系,以不同浓度(12.5、25、50和100μmol/L)、不同作用时间(24、48、72和96h)对原头节进行药物(Smad蛋白抑制剂SIS3)干预试验,伊红染色和扫描及透射电镜观察不同浓度药物干预过程中原头节活力及虫体超微结构的变化。结果SIS3干预48h,50和100μmol/L SIS3组与空白对照组原头节活力显著下降;作用96h,50和100μmol/L SIS3组原头节活力分别下降(10.06±2.79)%和(19.49±2.63)%。随着药物浓度的增加,扫描电镜观察原头节体表绒毛排列紊乱,吸盘结构变形,顶突小钩脱落;透射电镜观察药物组原头节表层微毛减少或消失,合胞体带变薄,胞浆空泡化,虫体内部出现脂滴等。结论 Smad信号通路抑制剂SIS3对体外细粒棘球蚴有抑制作用,为治疗细粒棘球蚴病提供了理论基础。
[Abstract]:Objective to study the effect of Smad protein inhibitor SIS3 on the procephalic ganglia of Echinococcus granulosus in vitro. Methods the culture system of Echinococcus granulosus in vitro was established. Different concentrations of Echinococcus granulosus were treated with different concentrations (12.5, 25, 50 and 100 渭 mol / L), for 24, 48, 72 and 96 hours). The drug (Smad protein inhibitor SIS3) was used to interfere with Echinococcus granulosus. The changes of the activity of procephalic ganglia and the ultrastructure of parasite during different concentrations of drug intervention were observed by eosin staining, scanning electron microscope and transmission electron microscope. Results after SIS3 intervention for 48 h, the activity of primary head node in 50 渭 mol / L SIS3 group and blank control group decreased significantly, and that in 50 渭 mol / L SIS3 group and 100 渭 mol / L SIS3 group decreased by (10.06 卤2.79)% and (19.49 卤2.63)% at 96 h, respectively. With the increase of drug concentration, the arrangement of villi on the surface of the original head node was disordered, the structure of the sucker was deformed and the hook of the apical process fell off with the increase of the concentration of the drug. Transmission electron microscope showed that in the drug group, the microhair on the surface of the original head decreased or disappeared, the syncytial zone became thinner, the cytoplasm was vacuolated, and lipid droplets appeared in the insect body. Conclusion SIS3, an inhibitor of Smad signaling pathway, has inhibitory effect on Echinococcus granulosus in vitro, which provides a theoretical basis for the treatment of Echinococcus granulosus.
【作者单位】： 新疆医科大学基础医学院;新疆医科大学第一附属医院/临床医学研究院 新疆包虫病基础医学重点实验室;
【基金】：新疆维吾尔自治区自然科学基金项目(No.2015211C029) 新疆医科大学大学生创新性实验计划项目基金项目(No.国家级201510760019) 新疆重大疾病医学重点实验室开放课题项目(No.SKLIB-XJMDR-2016-3)
【分类号】：R383.33
，
本文编号：2479609
[Abstract]:Objective to study the effect of Smad protein inhibitor SIS3 on the procephalic ganglia of Echinococcus granulosus in vitro. Methods the culture system of Echinococcus granulosus in vitro was established. Different concentrations of Echinococcus granulosus were treated with different concentrations (12.5, 25, 50 and 100 渭 mol / L), for 24, 48, 72 and 96 hours). The drug (Smad protein inhibitor SIS3) was used to interfere with Echinococcus granulosus. The changes of the activity of procephalic ganglia and the ultrastructure of parasite during different concentrations of drug intervention were observed by eosin staining, scanning electron microscope and transmission electron microscope. Results after SIS3 intervention for 48 h, the activity of primary head node in 50 渭 mol / L SIS3 group and blank control group decreased significantly, and that in 50 渭 mol / L SIS3 group and 100 渭 mol / L SIS3 group decreased by (10.06 卤2.79)% and (19.49 卤2.63)% at 96 h, respectively. With the increase of drug concentration, the arrangement of villi on the surface of the original head node was disordered, the structure of the sucker was deformed and the hook of the apical process fell off with the increase of the concentration of the drug. Transmission electron microscope showed that in the drug group, the microhair on the surface of the original head decreased or disappeared, the syncytial zone became thinner, the cytoplasm was vacuolated, and lipid droplets appeared in the insect body. Conclusion SIS3, an inhibitor of Smad signaling pathway, has inhibitory effect on Echinococcus granulosus in vitro, which provides a theoretical basis for the treatment of Echinococcus granulosus.
【作者单位】： 新疆医科大学基础医学院;新疆医科大学第一附属医院/临床医学研究院 新疆包虫病基础医学重点实验室;
【基金】：新疆维吾尔自治区自然科学基金项目(No.2015211C029) 新疆医科大学大学生创新性实验计划项目基金项目(No.国家级201510760019) 新疆重大疾病医学重点实验室开放课题项目(No.SKLIB-XJMDR-2016-3)
【分类号】：R383.33
，
本文编号：2479609
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