金口宝对兔口腔溃疡模型的影响

发布时间：2019-05-26 21:30

【摘要】：目的探讨金口宝对兔口腔溃疡的治疗效果及其作用机制。方法在60只实验用SPF级新西兰大白兔中随机抓取6只作为溃疡模型的鉴定,其余随机等分为3组:对照组(NC组)、生理盐水药膜组(NS组)、金口宝药膜组(JK组)。用40%的冰醋酸溶液烧灼兔口腔颊膜复制口腔溃疡模型。观察在造模当天及给药的第3、5、7天兔口腔溃疡的变化情况,利用逆转录PCR检测兔口腔颊部黏膜组织中表皮生长因子(EGF)水平,使用HE染色观察兔口腔溃疡局部病理组织学变化。结果与NS组比较,在给药的第3、5、7天JK组可以显著减小溃疡面积(P0.01)。与NC组比较,NS组、JK组兔口腔颊部黏膜组织中EGF水平明显升高(P0.01),但JK组EGF水平较NS组升高快,两组比较差异有统计学意义(P0.01)。在给药的第3、5、7天,兔口腔溃疡HE染色切片显示,JK组较NS组炎症细胞明显减少,成纤维细胞明显增生,上皮增生情况良好。结论金口宝可以明显改善兔口腔溃疡症状并促进溃疡愈合,金口宝可能是通过调节口腔溃疡时EGF水平而增强口腔溃疡的修复能力。
[Abstract]:Objective to investigate the therapeutic effect and mechanism of Jinkoubao on oral ulcer in rabbits. Methods six of 60 SPF New Zealand white rabbits were randomly selected as ulcer models. The others were randomly divided into three groups: control group (NC group), saline membrane group (NS group) and Jinkoubao drug membrane group (JK group). The model of oral ulcer was established by burning rabbit buccal membrane with 40% acetic acid solution. The changes of oral ulcer in rabbits on the day of modeling and on the 3rd, 5th and 7th day of administration were observed. The level of epidermal growth factor (EGF) in oral and buccal mucosa of rabbits was detected by reverse transcription PCR (RT-PCR). The local pathological changes of oral ulcer in rabbits were observed by HE staining. Results compared with NS group, on the 3rd and 5th day of administration, JK group could significantly reduce the ulcer area on the 3rd and 7th day (P 0.01). Compared with NC group, the level of EGF in oral and buccal mucosa of rabbits in NS group and JK group was significantly higher than that in NS group (P 0.01), but the level of EGF in JK group was faster than that in NS group, and there was significant difference between the two groups (P 0.01). On the 3rd, 5th and 7th day after administration, HE staining sections of oral ulcer in rabbits showed that the number of inflammatory cells, fibroblasts and epithelial hyperplasia in JK group were significantly lower than those in NS group. Conclusion Jinkoubao can significantly improve the symptoms of oral ulcer and promote ulcer healing in rabbits. Jinkoubao may enhance the repair ability of oral ulcer by regulating the level of EGF in oral ulcer.
【作者单位】：西南医科大学附属医院护理部;西南医科大学护理学院;西南医科大学附属医院肝胆外科;西南医科大学附属医院血液内科;
【基金】：四川省科技厅(苗子工程)资助项目[川科发计(2014)10号]
【分类号】：R285.5;R-332

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