桂枝汤对糖尿病模型大鼠心脏自主神经重塑的影响

发布时间：2019-05-26 22:40

【摘要】：目的观察桂枝汤对糖尿病大鼠心脏自主神经病变重塑的影响及可能作用机制。方法 24只大鼠随机分为正常对照组、模型对照组、弥可保组和桂枝汤组,每组8只。除正常对照组外,其余大鼠采用一次性腹腔注射链脲佐菌素建立糖尿病模型。弥可保组给予弥可保混悬液0.15 mg/(kg·d)灌胃,桂枝汤组给予桂枝汤4 g/(kg·d)灌胃,正常对照组和模型对照组大鼠各给予等量生理盐水灌胃,各组均灌胃7周。采用生理参数遥测系统检测大鼠的心电图并计算心率变异性(HRV),包括时域分析的平均正常RR间期的标准差(SDNN)、相邻RR间期差的均方根(RMSSD)、每5min正常RR间期平均值的标准差(SDANN)和频域分析的低频(LF)、高频(EF)、总功率(TP)及LF/EF;检测室间隔和左心室心肌匀浆中酪氨酸羟化酶(TH)、胆碱乙酰氨基转移酶(Ch AT)、生长相关蛋白43(GAP-43)、神经营养因子(NGF)和睫状神经营养因子(CNTF)含量。结果与模型对照组比较,桂枝汤组大鼠SDNN、RMSSD和LF、HF、TP增高,而SDANN和LF/HF降低;大鼠室间隔和左心室Ch AT增加、TH/Ch AT降低,室间隔和左心室GAP-43、CNTF增加,而室间隔NGF降低(P0.05或P0.01)。与弥可保组比较,桂枝汤大鼠SDANN和LF/HF降低,RMSSD、LF、HF和TP升高;大鼠室间隔和左心室Ch AT、GAP-43、CNTF增加,TH/Ch AT降低(P0.05或P0.01)。结论桂枝汤可预防糖尿病大鼠心脏自主神经损伤,减轻自主神经重塑,其机制可能与调节交感神经和迷走神经平衡有关。
[Abstract]:Objective to observe the effect of Guizhi decoction on cardiac autonomic neuropathy remodeling in diabetic rats and its possible mechanism. Methods Twenty-four rats were randomly divided into normal control group, model control group, Mikebao group and Guizhi decoction group with 8 rats in each group. In addition to the normal control group, the diabetic model was established by intraabdominal injection of streptozotocin in other rats. The rats in the Mikebao group were given 0.15 mg/ (kg 路d) suspension, the Guizhi decoction group was given 4 g / (kg 路d) Guizhi decoction, and the rats in the normal control group and the model control group were given the same amount of saline. All groups were given intragastrically for 7 weeks. The electrocardiogram of rats was measured by physiological parameter telemetry system and the root mean square (RMSSD), of (SDNN), adjacent RR interval difference was calculated by heart rate variability (HRV), including the standard deviation of normal RR interval analyzed in time domain. Standard deviation (SDANN) for the average value of normal RR interval per 5min and low Frequency (LF), High Frequency (EF), Total Power (TP) and LF/EF; analyzed in Frequency Domain The contents of (TH), cholinylaminotransferase (Ch AT), growth associated protein 43 (GAP-43), neurotrophic factor (NGF) and ciliary neurotrophic factor (CNTF) in interventricular septum and left ventricular myocardial homogenate were measured. Results compared with the model control group, SDNN,RMSSD and LF,HF,TP increased, but SDANN and LF/HF decreased in Guizhi decoction group. The Ch AT of interventricular septum and left ventricle increased, TH/Ch AT decreased, GAP-43,CNTF of interventricular septum and left ventricle increased, while NGF of interventricular septum decreased (P 0.05 or P 0.01). Compared with Mikebao group, SDANN and LF/HF decreased, RMSSD,LF,HF and TP increased, Ch AT,GAP-43,CNTF and TH/Ch AT decreased in interventricular septum and left ventricle of rats with Guizhi decoction (P 0.05 or P 0.01). Conclusion Guizhi decoction can prevent cardiac autonomic nerve injury and alleviate autonomic nerve remodeling in diabetic rats, and its mechanism may be related to the regulation of sympathetic nerve and vagus nerve balance.
【作者单位】：山东中医药大学附属医院;山东中医药大学;
【基金】：国家自然科学基金(81072962)
【分类号】：R285.5;R-332

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