抑制Ppif基因的表达减轻大鼠肾缺血再灌注损伤

发布时间：2019-06-01 17:23

【摘要】：目的通过抑制Ppif基因的表达,观察其对实验大鼠缺血再灌注损伤肾脏所起的保护效果,并分析、讨论其作用原理。方法首先用大鼠建立肾缺血再灌注模型,并随机将实验大鼠分组(共3组):空白对照组(NC Group)、阴性对照组(CON Group)、治疗组(Treated Group)(每组各20只大鼠)。治疗组大鼠再灌注时注射Ppif基因靶向RNAi慢病毒载体,剂量0.3 mL(4μg/ g),阴性对照组再灌注时注射0.3 mL生理盐水(含体积分数0.01 DMSO,即二甲基亚砜),空白对照组不处理肾蒂,即肾脏未经历缺血再灌注,其余处理措施按阴性对照组方法进行。达预定时间后,分别用相应方法检测各实验组大鼠细胞凋亡指数(AI)、血肌酐(Cr)水平、血尿素氮(BUN)水平、HE染色细胞病理切片检查。结果治疗组大鼠相对于阴性对照组大鼠,血液中Cr和BUN测定数值均明显下降,AI明显减低,二者的差异均有统计学意义(P 0.05)。病理学检查结果(HE组织染色方法)显示:3组大鼠对比,治疗组大鼠缺血明显减轻。结论大鼠Ppif基因的表达能够被Ppif基因靶向RNAi慢病毒载体有效抑制,从而达到抑制线粒体途径凋亡的目的,最终当大鼠肾脏缺血再灌注出现时,起到对缺血肾脏有效的保护作用。
[Abstract]:Objective to observe the protective effect of Ppif gene on renal ischemia-reperfusion injury in rats by inhibiting the expression of VEGF gene, and to analyze and discuss its principle of action. Methods the model of renal ischemia-reperfusion was established by using rats, and the experimental rats were randomly divided into three groups: blank control group (NC Group), negative control group (CON Group), treatment group (Treated Group) (each group 20 rats). The rats in the treatment group were injected with Ppif gene targeting RNAi lentivirus vector at a dose of 0.3 mL (4 渭 g / g), negative control group). 0.3 mL saline (containing volume fraction 0.01 DMSO, dimethyl sulfoxide) was injected into the treatment group. The blank control group did not deal with the renal pedicle, that is, the kidney did not experience ischemia-reperfusion, and the other treatment measures were carried out according to the negative control group. After reaching the predetermined time, the apoptosis index (AI), serum creatinine (Cr) level, blood urea nitrogen (BUN) level and HE staining cell pathological section were measured by corresponding methods. Results compared with the negative control group, the values of Cr and BUN in the blood of the treatment group were significantly lower than those of the negative control group, and the AI of the treatment group was significantly lower than that of the negative control group (P 0.05). The results of pathological examination (HE tissue staining) showed that the ischemia of the rats in the treatment group was significantly alleviated compared with that in the three groups. Conclusion the expression of Ppif gene in rats can be effectively inhibited by Ppif gene targeting RNAi lentivirus vector, so as to inhibit the apoptosis of mtDNA pathway, and finally play an effective protective effect on ischemic kidney when ischemia-reperfusion occurs in rat kidney.
【学位授予单位】：华中科技大学
【学位级别】：硕士
【学位授予年份】：2011
【分类号】：R363

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