种子育麟散对超促排卵模型大鼠子宫内膜整合素αvβ3、HOXA10表达的影响
发布时间：2019-07-01 13:16
【摘要】：目的 探讨种子育麟散治疗不孕症的可能作用机制。方法 选取30只SD雌性大鼠随机分为空白对照组、模型组、中药组,每组10只。模型组和中药组通过腹腔内注射丙氨瑞林、尿促性腺激素、注射用绒毛膜促性腺激素建立控制性超促排卵模型大鼠。造模开始的同时中药组给予种子育麟散7.2 g/(kg·d)灌胃,模型组、空白对照组给予等量生理盐水灌胃,均每日1次,每次2 ml,连续9天。于孕5天后分别采用免疫组化法、Western blot法检测大鼠子宫内膜整合素αvβ3、同源框(HOX)A10蛋白表达,RT-PCR法检测HOXA10、整合素αvβ3 mRNA表达。结果 与空白对照组比较,模型组子宫内膜整合素αvβ3、HOXA10蛋白及HOXA10、整合素αvβ3 mRNA表达量明显减少(P0.05);与模型组比较,中药组子宫内膜整合素αvβ3、HOXA10蛋白及整合素αvβ3、HOXA10 mRNA表达量明显增加(P0.05)。结论 种子育麟散能够提高大鼠子宫内膜整合素αvβ3、HOXA10蛋白及mRNA的表达,从而改善妊娠结局,可能是其治疗不孕症的机制之一。
[Abstract]:Objective to explore the possible mechanism of seed Yulin Powder in the treatment of sterility. Methods 30 female SD rats were randomly divided into blank control group (n = 10), model group (n = 10) and traditional Chinese medicine group (n = 10). In the model group and the traditional Chinese medicine group, the controlled superovulatory model rats were established by intraabdominal injection of propyrine, urinary gonadotropin and chorionic gonadotropin. At the beginning of the model, the traditional Chinese medicine group was given seed Yulin Powder 7.2g / (kg 路d) intragastrically, and the model group and the blank control group were given the same amount of saline once a day for 2 ml, for 9 days. After 5 days of pregnancy, the expression of integrin 伪 v 尾 3 and homeobox (HOX) A10 protein in rat endometrium was detected by, Western blot method, and the expression of HOXA10, integrin 伪 v 尾 3 mRNA was detected by RT-PCR assay. Results compared with the blank control group, the expression of integrin 伪 v 尾 3, HoxA10 protein and HOXA10, integrin 伪 v 尾 3 mRNA in the model group was significantly lower than that in the model group (P 0.05). Compared with the model group, the expression of integrin 伪 v 尾 3, HOXA10 protein and integrin 伪 v 尾 3, HOXA10 mRNA increased significantly in the model group (P 0.05). Conclusion seed Yulin Powder can increase the expression of integrin 伪 v 尾 3, HoxA10 protein and mRNA in rat endometrium, thus improving the pregnancy outcome, which may be one of the mechanisms of its treatment of sterility.
【作者单位】： 杭州市第一人民医院/南京医科大学附属杭州医院;
【分类号】：R285.5;R-332
[Abstract]:Objective to explore the possible mechanism of seed Yulin Powder in the treatment of sterility. Methods 30 female SD rats were randomly divided into blank control group (n = 10), model group (n = 10) and traditional Chinese medicine group (n = 10). In the model group and the traditional Chinese medicine group, the controlled superovulatory model rats were established by intraabdominal injection of propyrine, urinary gonadotropin and chorionic gonadotropin. At the beginning of the model, the traditional Chinese medicine group was given seed Yulin Powder 7.2g / (kg 路d) intragastrically, and the model group and the blank control group were given the same amount of saline once a day for 2 ml, for 9 days. After 5 days of pregnancy, the expression of integrin 伪 v 尾 3 and homeobox (HOX) A10 protein in rat endometrium was detected by, Western blot method, and the expression of HOXA10, integrin 伪 v 尾 3 mRNA was detected by RT-PCR assay. Results compared with the blank control group, the expression of integrin 伪 v 尾 3, HoxA10 protein and HOXA10, integrin 伪 v 尾 3 mRNA in the model group was significantly lower than that in the model group (P 0.05). Compared with the model group, the expression of integrin 伪 v 尾 3, HOXA10 protein and integrin 伪 v 尾 3, HOXA10 mRNA increased significantly in the model group (P 0.05). Conclusion seed Yulin Powder can increase the expression of integrin 伪 v 尾 3, HoxA10 protein and mRNA in rat endometrium, thus improving the pregnancy outcome, which may be one of the mechanisms of its treatment of sterility.
【作者单位】： 杭州市第一人民医院/南京医科大学附属杭州医院;
【分类号】：R285.5;R-332
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