归脾汤联合氟西汀对抑郁模型大鼠行为学及海马区NE、5-HT及DA的影响研究

发布时间：2019-07-12 10:57

【摘要】：目的:研究归脾汤对慢性不可预测温和刺激所致抑郁模型大鼠的行为学及海马5-HT、NE、DA含量的影响。方法:选取雌性成年SD大鼠60只,随机分为5组,即正常对照组、模型组、归脾汤组、氟西汀组、归脾汤+氟西汀组,每组12只。建立慢性不可预见性温和应激抑郁模型后分别给予相应药物干预,比较各组大鼠行为学及海马NE、S-HT、DA含量的影响。结果:与正常对照组比较,实验4、6、8 w,模型组大鼠体质量增长显著降低,爬格及直立次数得分显著降低,糖水消耗量显著减少,糖水偏爱比率显著降低,强迫游泳不动时间显著延长(P0.05)。与模型组比较,各给药组抑郁相关症状逐步改善,6、8 w各给药组体质量增长显著升高,精神状态改善,爬格及直立次数得分及糖水消耗量显著增加,糖水偏爱比率显著升高,强迫游泳不动时间显著缩短(P0.05)。实验8 w末,与正常对照组比较,模型组海马组织5-HT、NE、DA含量显著降低(P0.05),与模型组比较,各给药组海马组织5-HT、NE、DA含量显著升高(P0.05)。结论:归脾汤可改善大鼠抑郁症状,其机制可能与增加大脑海马5-HT、DA及NE的含量有关。归脾汤与氟西汀联用后效果更佳。
[Abstract]:Objective: To study the effect of Gui-Pi-Tang on the behavior and the content of 5-HT, NE and DA in the rats with depression model caused by chronic unpredictable mild-stimulation. Methods:60 female adult SD rats were randomly divided into 5 groups, namely, the normal control group, the model group, the spleen decoction group, the fluoxetine group, the spleen-spleen decoction and the fluoxetine group, and 12 rats in each group. The effects of the behavior of the rats and the content of NE, S-HT and DA in the hippocampus were compared after the model of chronic unpredictability and stress and depression were established. Results: Compared with the normal control group, the body mass increased significantly in the experimental group 4,6 and 8 w, the score of the number of climbing and the number of upright times decreased significantly, the consumption of sugar water was significantly reduced, the preference ratio of sugar water was significantly reduced, and the time of forced swimming was significantly prolonged (P0.05). Compared with the model group, the symptoms of depression in each treatment group were gradually improved, and the quality of the 6 and 8w administration groups increased significantly, the mental state was improved, the scores of the number of climbing and the number of upright times and the consumption of the sugar water were significantly increased, and the preference ratio of the sugar water was significantly increased. The time of forced swimming was significantly shortened (P0.05). At the end of 8w, the content of 5-HT, NE and DA in the hippocampus of the model group was significantly lower than that of the normal control group (P0.05). The content of 5-HT, NE and DA in the hippocampus of the model group was significantly higher than that of the model group (P0.05). Conclusion: It can improve the depressive symptoms of the rat, and the mechanism may be related to the increase of the content of 5-HT, DA and NE in the brain of the brain. The effect of the combination of Gui-Pi-Tang and fluoxetine is better.
【作者单位】：杭州市第七人民医院;
【基金】：浙江省中医药管理局项目(2012ZB134) 杭州市卫生局医药卫生项目(2012A039,2014A048)
【分类号】：R285.5;R-332

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