抗猪囊尾蚴IgG4抗体的特异性抗原筛选

发布时间：2019-07-20 05:37

【摘要】：将囊液抗原蛋白采用双向凝胶电泳分离,经考马斯亮蓝染色后用双向电泳图像分析软件分析蛋白点,以脑囊尾蚴病患者血清为一抗进行蛋白质印迹分析(Western blotting),选取阳性蛋白点进行质谱分析,获取肽质量指纹图谱,采用Mascot软件在NCBI中搜寻、比对,筛选出抗猪囊尾蚴IgG4特异性抗原。囊液抗原经双向电泳检测到(201±5)个蛋白斑点,相对分子质量(M_r)为10 000~170 000,等电点(pI)为3.0~10.0。Western blotting分析显示,与患者血清中特异IgG4抗体结合的抗原以小分子量蛋白为主,从中筛选出51个(抗原)点进行质谱分析,其中有21个点峰值大于39。对比分析显示,高峰值点与10种囊尾蚴蛋白有关,其中最为密切相关的为4种蛋白,按分值高低排序分别为Ts8B2、Ts8B3、10ku抗原和Sequence 3。
[Abstract]:The antigen protein of cysticercosis was separated by two-dimensional gel electrophoresis. After staining with Coomassie brilliant blue, the protein spots were analyzed by two-dimensional electrophoresis image analysis software. (Western blotting), positive protein spots were selected for mass spectrometry with serum of patients with cerebral cysticercosis as primary antibody for mass spectrometry analysis. The peptide mass fingerprint was obtained. Mascot software was used to search for IgG4 specific antigens against cysticercus cellulosae. The antigen of capsule fluid was detected by two-dimensional electrophoresis with (201 卤5) protein spots, the relative molecular weight (M 鈮，

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