复方川芎滴丸对糖尿病肾病模型大鼠血液流变学及血气的影响

发布时间：2019-07-25 06:57

【摘要】：目的:探讨复方川芎滴丸对糖尿病肾病(DN)模型大鼠血液流变学及血气的影响。方法:将60只大鼠随机分为正常对照组、模型组、阳性对照组[厄贝沙坦片,0.013 g/(kg·d)]和复方川芎滴丸高、中、低剂量组[0.4、0.2、0.1 g/(kg·d)],每组10只。除正常对照组外,其余各组大鼠均以高糖高脂饲料+ip链脲佐菌素的方法复制DN模型。成模7 d后,各给药组大鼠每天ig相应药物1次,10 m L/kg;正常对照组和模型组大鼠ig等体积纯化水。64 d后,测定大鼠血液流变学指标[全血黏度(高、中、低切)、血浆黏度、全血还原黏度]和血气指标(二氧化碳分压、氧分压、血红蛋白含量、氧饱和度、氧合血红蛋白占总蛋白的比例),苏木精-伊红染色观察大鼠肾组织病理变化。结果:与正常对照组比较,模型组大鼠血液流变学各指标和二氧化碳分压均明显升高(P0.05或P0.01),氧分压、血红蛋白含量、氧饱和度、氧合血红蛋白占总蛋白的比例均明显降低(P0.05或P0.01),肾组织损伤严重。与模型组比较,除复方川芎滴丸低剂量组大鼠二氧化碳分压、氧分压、血红蛋白含量改善不显著外,其余各给药组大鼠上述指标均显著改善(P0.05或P0.01),大鼠肾组织损伤也不同程度地减轻。结论:复方川芎滴丸可显著改善DN模型大鼠异常的血液流变学状态,降低血液黏度,改善微循环,提高血液运输氧的能力,且对DN大鼠的肾组织形态有一定的改善作用。
[Abstract]:Objective: to investigate the effect of compound Chuanxiong dropping pills on hemorheology and blood gas in (DN) rats with diabetic nephropathy. Methods: sixty rats were randomly divided into normal control group, model group, positive control group (0.013 g / (kg 路d) and compound Chuanxiong dropping pill high, middle and low dose group [0.4, 0.2, 0.1g / (kg 路d)] with 10 rats in each group. Except for the normal control group, the DN model was established by high glucose and high fat diet ip streptozotocin. After 7 days of modeling, the rats in each administration group were treated with ig once a day, 10 mL 路kg ~ (- 1) 路L ~ (- 1) 路kg ~ (- 1). After 64 days, the hemorheological indexes (whole blood viscosity (high, middle and low shear), plasma viscosity, whole blood reductive viscosity) and blood gas indexes (partial pressure of carbon dioxide, partial pressure of oxygen, hemoglobin content, oxygen saturation and the proportion of oxidized hemoglobin to total protein) were measured, and the pathological changes of renal tissue were observed by hematoxylin-eosin staining. Results: compared with the normal control group, the indexes of hemorheology and the partial pressure of carbon dioxide in the model group were significantly increased (P 0.05 or P 0.01), while the partial pressure of oxygen, hemoglobin content, oxygen saturation and the proportion of oxidized hemoglobin to total protein were significantly decreased (P 0.05 or P 0.01), and the injury of renal tissue was serious. Compared with the model group, except that the partial pressure of carbon dioxide, partial pressure of oxygen and the content of hemoglobin in the low dose group of compound Chuanxiong dropping pills were not significantly improved, the above indexes were significantly improved in the other administration groups (P 0.05 or P 0.01), and the injury of renal tissue was also alleviated in varying degrees. Conclusion: compound Chuanxiong dropping pills can significantly improve the abnormal hemorheological state, decrease the blood viscosity, improve the microcirculation and improve the ability of blood oxygen transport in DN model rats, and can improve the renal tissue morphology of DN rats to a certain extent.
【作者单位】：解放军第88医院药剂科;
【分类号】：R285.5;R-332

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