刺糖多糖对S180荷瘤小鼠动物模型肿瘤生长的影响研究

发布时间：2019-08-10 12:59

【摘要】：目的观察刺糖多糖及异常黑胆质成熟剂(ASMq)对S180荷瘤小鼠动物模型生长的影响。方法建立S180荷瘤小鼠动物模型,采用体内试验方法,分别检测刺糖多糖与其复方ASMq对S180实体瘤小鼠抑瘤率及相关脏器指数的影响;采用酶联免疫实验(ELISA)对小鼠血清中肿瘤坏死因子α(TNF-α)和干扰素γ(IFN-γ)含量进行检测。结果与对照组比较,刺糖多糖与其复方ASMq同时具有抑制肿瘤生长的作用,差异有统计学意义(P0.05),以刺糖低剂量组的抑瘤作用更为明显。与对照组比较,刺糖多糖与其复方ASMq均可提高荷瘤小鼠血清TNF-α、IFN-γ的含量,差异有统计学意义(P0.05)。结论刺糖多糖及其复方ASMq可抑制荷瘤小鼠瘤块的生长,能促进荷瘤小鼠免疫细胞分泌TNF-α。
[Abstract]:Objective to observe the effects of glycosaminoglycan and abnormal nigrocholic mature agent (ASMq) on the growth of S180 tumor-bearing mice. Methods the animal model of S180 tumor-bearing mice was established, and the effects of polysaccharide and its compound ASMq on the tumor inhibition rate and related organ index of S180 solid tumor mice were detected by in vivo test, and the contents of tumor necrosis factor-伪 (TNF- 伪) and interferon-gamma (IFN- 纬) in serum of mice were detected by enzyme-linked immunosorbent assay (ELISA). Results compared with the control group, Scutellaria polysaccharide and its compound ASMq could inhibit tumor growth at the same time, the difference was statistically significant (P 0.05), especially in the low dose group. Compared with the control group, both spiny polysaccharide and its compound ASMq could increase the contents of TNF- 伪 and IFN- 纬 in serum of tumor-bearing mice, the difference was statistically significant (P 0.05). Conclusion Sugar polysaccharide and its compound ASMq can inhibit the growth of tumor mass in tumor-bearing mice and promote the secretion of TNF- 伪 by immune cells in tumor-bearing mice.
【作者单位】：新疆医科大学第一附属医院医学检验中心;新疆医科大学;新疆医科大学药学院;
【基金】：国家自然科学基金(No.81460633) 新疆维吾尔自治区科技援疆计划(No.201491172)
【分类号】：R285.5;R-332

【相似文献】