当归饮子对银屑病模型豚鼠神经酰胺含量、AQP-3基因及蛋白表达的干预研究
发布时间：2019-08-13 14:54
【摘要】：目的探讨当归饮子对寻常型银屑病模型豚鼠皮肤神经酰胺(ceramide,Cer)含量、水通道蛋白3(aquaporin 3,AQP-3)基因及蛋白表达的干预影响。方法建立寻常型银屑病动物模型,分为当归饮子组、甲氨蝶呤组、生理盐水组、模型对照组、空白对照组,每组8只,酶联免疫吸附实验(ELISA)法检测造模皮损部位Cer含量,逆转录PCR(RT-PCR)和免疫印迹试验(Western blot)法检测造模皮损部位AQP-3基因及蛋白表达,分析寻常型银屑病动物模型皮损与皮肤屏障功能密切相关的基因及蛋白表达的变化。结果豚鼠耳部皮肤银屑病模型造模成功。模型对照组皮损中ELISA法检测Cer含量,以及RT-PCR和Western blot法检测AQP-3基因的蛋白表达均较低;当归饮子组与甲氨蝶呤组和各组中Cer含量、AQP-3基因蛋白表达增强(P0.01),且当归饮子组较甲氨蝶呤组增强明显(P0.01)。结论当归饮子能增强银屑病模型豚鼠皮肤Cer含量及AQP-3基因和蛋白表达,对改善银屑病动物模型的皮肤屏障功能作用确切,效果优于甲氨蝶呤组。
[Abstract]:Objective to investigate the effect of Angelica sinensis decoction on the content of ceramide (ceramide,Cer) and the expression of aquaporin 3 (aquaporin 3) gene and protein in guinea pigs with psoriasis vulgaris. Methods the animal model of psoriasis vulgaris was established and divided into Angelica sinensis group, methotrexate group, saline group, model control group and blank control group with 8 rats in each group. The content of Cer in the lesion site was detected by enzyme-linked immunosorbent assay (ELISA), and the expression of AQP-3 gene and protein in the lesion site was detected by reverse transcription PCR (RT-PCR) and immunoblotting (Western blot). To analyze the changes of gene and protein expression in psoriatic vulgaris model, which are closely related to skin barrier function. Results the model of psoriasis in the ear of guinea pigs was successfully established. The content of Cer in the skin lesions of the model control group was detected by ELISA, and the protein expression of AQP-3 gene was detected by RT-PCR and Western blot. The content of Cer and the expression of AQP-3 gene protein in Angelica sinensis group and methotrexate group and each group were increased (P 0.01), and the expression of AQP-3 gene protein in Angelica sinensis group was significantly higher than that in methotrexate group (P 0.01). Conclusion Angelica sinensis decoction can enhance the content of Cer and the expression of AQP-3 gene and protein in the skin of psoriatic guinea pigs, and has a definite effect on improving the skin barrier function of psoriatic animal model, and the effect is better than that of methotrexate group.
【作者单位】： 新疆医科大学附属中医医院皮肤科;湖南中医药大学研究生院;湖南中医药大学第二附属医院中医外科皮肤科;
【基金】：湖南省科技计划(2011FJ3026)
【分类号】：R285.5;R-332
[Abstract]:Objective to investigate the effect of Angelica sinensis decoction on the content of ceramide (ceramide,Cer) and the expression of aquaporin 3 (aquaporin 3) gene and protein in guinea pigs with psoriasis vulgaris. Methods the animal model of psoriasis vulgaris was established and divided into Angelica sinensis group, methotrexate group, saline group, model control group and blank control group with 8 rats in each group. The content of Cer in the lesion site was detected by enzyme-linked immunosorbent assay (ELISA), and the expression of AQP-3 gene and protein in the lesion site was detected by reverse transcription PCR (RT-PCR) and immunoblotting (Western blot). To analyze the changes of gene and protein expression in psoriatic vulgaris model, which are closely related to skin barrier function. Results the model of psoriasis in the ear of guinea pigs was successfully established. The content of Cer in the skin lesions of the model control group was detected by ELISA, and the protein expression of AQP-3 gene was detected by RT-PCR and Western blot. The content of Cer and the expression of AQP-3 gene protein in Angelica sinensis group and methotrexate group and each group were increased (P 0.01), and the expression of AQP-3 gene protein in Angelica sinensis group was significantly higher than that in methotrexate group (P 0.01). Conclusion Angelica sinensis decoction can enhance the content of Cer and the expression of AQP-3 gene and protein in the skin of psoriatic guinea pigs, and has a definite effect on improving the skin barrier function of psoriatic animal model, and the effect is better than that of methotrexate group.
【作者单位】： 新疆医科大学附属中医医院皮肤科;湖南中医药大学研究生院;湖南中医药大学第二附属医院中医外科皮肤科;
【基金】：湖南省科技计划(2011FJ3026)
【分类号】：R285.5;R-332
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3 李淑琴;;当归饮子临床应用举例[J];河北中医;1989年05期
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