泥蚶多糖佐剂对日本血吸虫DNA疫苗效果影响的初步研究

发布时间：2017-12-26 21:29

本文关键词：泥蚶多糖佐剂对日本血吸虫DNA疫苗效果影响的初步研究　出处：《中国血吸虫病防治杂志》2014年06期 　论文类型：期刊论文

【摘要】：目的探讨泥蚶多糖佐剂对日本血吸虫DNA疫苗免疫效果的影响。方法将60只雌性BALB/c小鼠随机分为对照组、泥蚶多糖组、疫苗组、疫苗+佐剂组等4组,每组15只,分别皮下注射100μl PBS、100μg泥蚶多糖、100μg pc DNA3.1-Sj26GST血吸虫DNA疫苗、100μg pc DNA3.1-Sj26GST与等量泥蚶多糖佐剂的混合物。各组分别于实验开始时、第2周、第4周各免疫1次。末次免疫后第2周,以日本血吸虫尾蚴40±1条/只经腹部皮肤攻击感染小鼠。感染后6周剖杀小鼠,收集血清、肝脏及成虫,以ELISA法测定血清中特异性Ig G水平,计算减虫率及每克肝组织减卵率。结果感染后6周,疫苗组和疫苗+佐剂组小鼠血清中Ig G抗体水平分别为18.26±0.42 mg/ml和20.21±0.89 mg/ml,差异有统计学意义(P0.05),两组均显著高于对照组(P均0.05);疫苗组减虫率和减卵率分别为28.60%和35.84%,疫苗+佐剂组减虫率和减卵率分别为38.04%和49.74%,差异均有统计学意义(P均0.05),且均显著高于PBS对照组(P均0.01)。结论泥蚶多糖作为佐剂,对日本血吸虫DNA疫苗具有明显增效作用。
[Abstract]:Objective to investigate the effects of Polysaccharide on the immune effect of adjuvant tegillarcagranosa DNA vaccine of Schistosoma japonicum. Methods 60 female BALB/c mice were randomly divided into control group, tegillarcagranosa polysaccharide group, the vaccine group, vaccine + adjuvant group 4 groups, 15 rats in each group, were injected 100 L PBS, 100 g, 100 g tegillarcagranosa polysaccharide PC DNA3.1-Sj26GST DNA vaccine of Schistosoma, 100 g PC and DNA3.1-Sj26GST etc. the amount of Tegillarca polysaccharide adjuvant mixture. Each group was immunized 1 times at the beginning of the experiment, second weeks and fourth weeks respectively. Second weeks after the last immunization, 40 + 1 Schistosoma japonicum cercariae were attacked in mice by abdominal skin attack. 6 weeks after infection, the mice were killed and the serum, liver and adult were collected. The specific Ig G level in serum was measured by ELISA method, and the rate of worm reduction and the rate of egg reduction per gram of liver were calculated. Results 6 weeks after infection, Ig antibody level of G vaccine and vaccine + adjuvant group in serum of mice were 18.26 + 0.42 and 20.21 + 0.89 mg/ml mg/ml, the difference was statistically significant (P0.05), the two groups were significantly higher than those in control group (P < 0.05); vaccine group worm and egg reduction rates respectively. The 28.60% and 35.84% vaccine + adjuvant group, the worm and egg reduction rates were 38.04% and 49.74%, the differences were statistically significant (P 0.05), and were significantly higher than those in control group (PBS P 0.01). Conclusion polysaccharides from Tegillarca granosa as adjuvant has obvious synergistic effect on Schistosoma japonicum DNA vaccine.
【作者单位】：皖南医学院医学寄生虫学教研室;
【基金】：国家自然科学基金(81141083) 安徽省高校自然科学重点基金(KJ2014A270)
【分类号】：R392
【正文快照】： 血吸虫病是一种严重威胁人类健康的人兽共患寄生虫病。虽然我国血防工作取得了重大成就,但由于日本血吸虫保虫宿主多,钉螺分布面积广,尚不能从根本上阻断血吸虫病的流行和传播[1-2]。吡喹酮虽能有效治疗血吸虫病患者,但有研究报道曼氏血吸虫某些虫株可产生耐药性等问题[3-4]。

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