中国西北部两省区结核分枝杆菌临床分离株Spoligotyping分型初步分析

发布时间：2017-12-27 00:36

本文关键词：中国西北部两省区结核分枝杆菌临床分离株Spoligotyping分型初步分析　出处：《实用预防医学》2014年05期 　论文类型：期刊论文

【摘要】：目的初步了解新疆和甘肃结核分枝杆菌的基因型、主要流行型及其分布特点,比较两省区之间基因型分布。方法采用随机抽样的方法挑选新疆地区结核分枝杆菌临床分离株179株,甘肃地区结核分枝杆菌临床分离株176株,采用间隔寡核苷酸分型(Spoligotyping)技术对所收集的临床分离株进行分型研究,分析我国西北部相邻的两省区结核分枝杆菌的基因型分布、主要流行型及其地区特征。结果新疆地区菌株分为47个基因型,其中28个基因型为新的型别,新疆菌株中北京家族占68.72%(123/179),其次为T家族(6.70%,12/179),H家族(3.91%,7/179)和CAS家族(2.23%,4/179);甘肃地区菌株分为29种基因型,其中10个基因型为新的型别,甘肃菌株中北京家族占86.93%(153/176),其次为T家族(4.55%,8/176),H家族(1.14%,2/176),MANU2(1.14%,2/176)和CAS(0.57%,1/176)。结论北京基因型菌株在新疆和甘肃地区均为主要流行株,但甘肃地区北京基因型菌株比例明显高于新疆地区。
[Abstract]:Objective to understand the genotypes, main epidemic types and distribution characteristics of Mycobacterium tuberculosis in Xinjiang and Gansu, and to compare the genotype distribution between the two provinces. Method to select the Xinjiang area 179 clinical isolated strains of Mycobacterium tuberculosis strains by using random sampling method, 176 strains of isolates of Mycobacterium tuberculosis in Gansu clinic, with spacer oligonucleotide typing (Spoligotyping) genotyping technology to collect the clinical isolates, the distribution and characteristics of main epidemic type and genotype analysis of China's northwest region the Ministry of the two adjacent provinces of Mycobacterium tuberculosis. The results of the Xinjiang area could be divided into 47 genotypes, including 28 genotypes for the new type of Xinjiang in Beijing family strains accounted for 68.72% (123/179), followed by the family of T (6.70%, 12/179), H family (3.91%, 7/179) and CAS family (2.23%, 4/179); the Gansu area is divided into 29 strains genotypes, including 10 genotypes for the new type of Gansu in Beijing family strains accounted for 86.93% (153/176), followed by the family of T (4.55%, 8/176), H family (1.14%, 2/176), MANU2 (1.14%, 2/176) and CAS (0.57%, 1/176). Conclusion the Beijing genotypic strains are the main epidemic strains in Xinjiang and Gansu, but the proportion of the genotype strains in Beijing of Gansu is obviously higher than that of Xinjiang.
【作者单位】：传染病预防控制国家重点实验室中国疾病预防控制中心传染病预防控制所;感染性疾病诊治协同创新中心;北京市朝阳区疾病预防控制中心;甘肃省兰州市肺科医院;新疆胸科医院;
【基金】：国家“艾滋病和病毒性肝炎等重大传染病防治科技重大专项”课题(2013ZX10003006-002-001)
【分类号】：R378
【正文快照】： 时至今日,结核病(tuberculosis,TB)这一古老的疾病仍然严重威胁人类的健康,成为全球的公共卫生问题之一。2011年全球新增病例870万,死亡病例140万[1]。中国作为全球22个高负担国家之一,目前在全国各省市仍然存在不同程度的结核发病率和流行情况。新疆维吾尔族自治群和甘肃省是

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