探索Sertoli细胞对去除精原细胞的动态反应

发布时间：2017-12-27 23:10

本文关键词：探索Sertoli细胞对去除精原细胞的动态反应　出处：《中国实验动物学报》2014年05期 　论文类型：期刊论文

【摘要】：目的探索Sertoli细胞对去除小鼠精原细胞后睾丸的动态反应。方法采用15、30和44 mg/kg的白消安腹腔注射法建立不同程度去除精原细胞的动物模型,处理后5 d和28 d时对睾丸进行组织学检测,评价精子发生状态,并运用实时定量荧光PCR技术检测这两个时期睾丸GDNF、PLZF、Nanog和GFRα1基因mRNA的表达量。结果在白消安处理后第5天,GDNF出现显著升高,且呈剂量依赖趋势,而PLZF与GFRɑ1并无显著变化,睾丸组织学观察亦无明显变化。在白消安处理后28 d时,GDNF、PLZF、Nanog、GFRɑ1基因mRNA相对表达量均出现大幅度的升高,睾丸组织学切片观察显示随着给药剂量的增加,精子发生受到的损伤愈加严重。结论 Sertoli细胞早在白消安处理后第5天就对精原细胞的变化发生了反应,Sertoli细胞分泌GDNF的能力发生代偿性增加,进而刺激精原干细胞自我更新速度加快,体现在Nanog和PLZF水平提高,从而实现精子发生的重建。
[Abstract]:Objective to explore the dynamic response of Sertoli cells to the testicle of spermatogonial cells in mice. Methods 15, 30 and 44 mg/kg by intraperitoneal injection of busulfan to establish different degree of removal of spermatogonia in animal models, after the treatment of 5 D and 28 d on testicular histological detection, evaluation of spermatogenesis, and the use of real-time quantitative PCR technique to detect the expression of the two stages of testis GDNF, PLZF Nanog and GFR, the amount of mRNA alpha 1 gene. Results fifth days after busulfan treatment, GDNF increased significantly in a dose dependent trend, while PLZF and GFR 1. There is no significant change in histological observation, there was no obvious change. In the busulfan treatment after 28 d, GDNF, PLZF, Nanog, GFR alpha 1 gene mRNA expression were increased significantly, the histological section showed that with the increase of the dose, the more serious damage of spermatogenesis by. Conclusion Sertoli cells in busulfan treatment fifth days after the change of spermatogonia reaction, generating capacity of Sertoli cell secretory GDNF compensatory increase, and thus stimulate the spermatogonial stem cell self-renewal speed reflects the enhancement in Nanog and PLZF level, so as to realize the reconstruction of spermatogenesis.
【作者单位】：首都师范大学生命科学学院;
【基金】：国家自然科学基金(No.30870934)
【分类号】：R339.21
【正文快照】： baochang@cnu.edu.cn精原干细胞(SSCs)位于曲细精管基底膜附近,是精子发生过程中各级生殖细胞的基础来源。国内外许多研究都对其功能及调控进行了详细研究[1-2],发现SSCs的自我更新及分化都受到睾丸微环境(niche)的调控[3],而对微环境贡献最直接的睾丸细胞应该是支持细胞(Sert

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