不同剂量STZ与高脂饲料联合诱导糖尿病小鼠模型

发布时间：2017-12-28 04:21

本文关键词：不同剂量STZ与高脂饲料联合诱导糖尿病小鼠模型　出处：《上海交通大学学报(农业科学版)》2014年06期 　论文类型：期刊论文

【摘要】：探讨不同剂量链脲霉素(streptozotocin,STZ)联合高脂膳食建立2型糖尿病小鼠模型的效果。选择C57BL/6J小鼠,5周龄开始饲喂高脂饲料,持续10周,将膳食诱导的肥胖(dietinduced obesity,DIO)小鼠随机分为对照组及3个试验组[分别为试验组A(腹腔注射STZ 50mg/kg)、B(腹腔注射STZ 70mg/kg)、C(腹腔注射STZ 100mg/kg)],每组均为10只动物。试验组小鼠腹腔注射STZ后与对照组一起继续饲喂高脂2周。分别检测各组小鼠造模前后空腹血糖,胰岛素水平及成模后糖耐量。结果表明试验A、B、C 3组的成模率分别为70%、90%、100%,其血糖含量(分别为229.4±8.72、349.4±6.71、432.5±11.0 mg/dL)显著地高于对照组(168.7±4.18mg/dL);其血浆胰岛素含量(分别为1.83±0.288,0.659±0.058,0.438±0.023ng/mL)明显低于对照组(2.95±0.218ng/mL)。不同STZ剂量可诱导不同程度的糖尿病症状。
[Abstract]:To explore the effect of different doses of streptozotocin (STZ) combined with high fat diet to establish the model of type 2 diabetes in mice. C57BL/6J mice, aged 5 weeks to fed with high fat diet for 10 weeks, the diet induced obesity (dietinduced, obesity, DIO) mice were randomly divided into control group and 3 experimental groups were as the test group A (intraperitoneal injection of STZ 50mg/kg (B), intraperitoneal injection of STZ 70mg/kg (C), intraperitoneal injection STZ 100mg/kg), 10 rats for each group animal. The mice in the experimental group were injected with STZ in the abdominal cavity and continued to feed high fat for 2 weeks with the control group. The fasting blood glucose, insulin level and the glucose tolerance after the model were measured before and after the model of the mice. The result shows that the test of A, B, C 3 group model was respectively 70%, 90% and 100%, the content of blood sugar (229.4 + 8.72, 349.4 + 6.71, 432.5 + 11 mg/dL) was significantly higher than the control group (168.7 + 4.18mg/dL); the plasma insulin level (1.83 + 0.288,0.659 + 0.058,0.438 + 0.023ng/mL) was significantly lower than the control group (2.95 + 0.218ng/mL). Different doses of STZ can induce different degrees of diabetes.
【作者单位】：上海交通大学农业与生物学院上海市兽医生物技术重点实验室;
【分类号】：R-332;R587.1
【正文快照】： 糖尿病(diabetes mellitus,DM)是遗传因素与环境因素共同作用的一组以高血糖为特征的代谢性疾病,主要由胰岛素作用抵抗和胰岛素分泌缺陷引起的糖、脂肪和蛋白质代谢紊乱[1]。除少数妊娠糖尿病和特殊类型糖尿病外,糖尿病主要分为两型:1型糖尿病和2型糖尿病。1型糖尿病的特征是

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