聚乙烯界面结核分枝杆菌黏附力及生物膜的形成

发布时间：2017-12-28 23:02

本文关键词：聚乙烯界面结核分枝杆菌黏附力及生物膜的形成　出处：《中国组织工程研究》2014年52期 　论文类型：期刊论文

【摘要】：背景:研究证实结核杆菌对钴铬钼、钛合金的黏附力低,且不形成生物膜,但其对聚乙烯的黏附力及生物膜形成情况未见报道。目的:观察利福平干预对聚乙烯界面结核杆菌黏附力及生物膜形成的影响。方法:将聚乙烯和钴铬钼实验材料在无菌条件下分别置于Middlebrook 7H9液体培养基中,与结核杆菌共同培养2周,随机取出2块后,再加入1 mg/L的利福平溶2 m L,继续培养2周后,用激光共聚焦显微镜观测利福平干预前后材料界面单位面积菌落数及菌落厚度,扫描电镜观察利福平干预前后材料界面细菌生物膜结构。结果与结论:利福平干预前,聚乙烯界面单位面积菌落数及菌落厚度均大于钴铬钼组(P0.05);利福平干预后,聚乙烯界面单位面积菌落数及菌落厚度较干预前明显减少(P0.05)。利福平干预前,聚乙烯界面可见结核杆菌黏附,形成似"雪花状"、"云雾状",可见生物膜结构;钴铬钼界面可见散在结核杆菌菌落,未见生物膜结构形成。利福平干预后,聚乙烯界面黏附菌落数减少,部分结核杆菌出现干瘪、裂解,生物膜有不同程度的破坏;钴铬钼界面未见菌落形成,偶见单个结核杆菌黏附,未见生物膜形成。表明结核杆菌可在聚乙烯界面形成生物膜,但利福平可抑制甚至破坏结核生物膜。
[Abstract]:Background: studies have confirmed that the adhesion force of Mycobacterium tuberculosis to cobalt chromium molybdenum and titanium alloy is low, and no biofilm is formed, but its adhesion to polyethylene and biofilm formation have not been reported. Objective: To observe the effect of rifampin intervention on the adhesion and biofilm formation of Mycobacterium tuberculosis at polyethylene interface. Methods: polyethylene and cobalt chromium molybdenum materials under aseptic conditions were placed in Middlebrook 7H9 liquid medium, and Mycobacterium tuberculosis co cultured for 2 weeks, were removed 2 after adding 1 mg/L of rifampicin solution for 2 m L, 2 weeks after cultured with laser scanning confocal microscopy observation before and after the intervention of rifampicin material the interface numbers of colonies per unit area and the thickness of colony were observed before and after the intervention, rifampicin material interface bacterial biofilm scanning electron microscope. Results and conclusion: before rifampicin intervention, the number of colony per unit area and colony thickness of polyethylene interface were all larger than those of cobalt chromium molybdenum group (P0.05). After rifampicin intervention, the number of colony per unit area and colony thickness of polyethylene interface decreased significantly compared with that before intervention (P0.05). Before rifampicin intervention, the adhesion of Mycobacterium tuberculosis could be seen at the polyethylene interface, forming a "snowflake" and "cloud like shape", showing biofilm structure. The co Cr Mo interface could be seen in the Mycobacterium tuberculosis colony, and no biofilm structure was formed. After rifampicin intervention, the number of interfacial adhesion bacteria decreased, and some Mycobacterium tuberculosis appeared dry and cracked, and the biofilms were damaged to varying degrees. There was no colony formation at the interface between cobalt, chromium and molybdenum. Occasionally, a single Mycobacterium tuberculosis adhered, and no biofilm formation was observed. It showed that the Mycobacterium tuberculosis could form the biofilm at the polyethylene interface, but rifampin could inhibit or even destroy the tuberculosis biofilm.
【作者单位】：天津医科大学第四中心临床学院;天津中医药大学;
【基金】：天津市卫生局科技基金项目(2013KY05)~~
【分类号】：R378.911
【正文快照】： 0引言Introduction骨与关节结核是常见的肺外结核,髋膝关节结核占25%-35%[1],其发病率逐年上升[2-4]。关节结核引起的疼痛、骨质破坏、功能受限等严重影响患者日常生活,给其治疗带来了严峻挑战,外科手术是解除痛苦、重建功能、提高生活质量的可靠方法。人工关节置换治疗活动性

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