人骨髓间充质干细胞单克隆抗体在骨髓和脐血间充质干细胞检测中的应用

发布时间：2017-12-31 02:19

本文关键词：人骨髓间充质干细胞单克隆抗体在骨髓和脐血间充质干细胞检测中的应用　出处：《浙江大学》2006年硕士论文　论文类型：学位论文

【摘要】：骨髓间充质干细胞(bone marrow mesenchymal stem cells,BM-MSCs)是不同于造血干细胞的另一类骨髓干细胞,具有自我更新、高度增殖和多向分化潜能,在一定的体内或体外诱导条件下,可以分化为脂肪细胞、软骨细胞、成骨细胞、星形胶质细胞和神经元细胞。最近研究表明间充质干细胞具有弱的免疫原性和很强的免疫调节功能,这些生物学特性使其在组织工程、基因治疗、细胞移植等领域具有广阔的应用前景。 20世纪90年代起研究者就试图寻找BM-MSCs特异性的表面标记,并通过杂交瘤技术制备了多株人MSCs相关的单克隆抗体,如SH2、SH3、SH4、STRO-1等,但这些单克隆抗体均与其它细胞存在交叉反应,故到目前为止,尚未发现人MSCs单一的特异性表面标记。在MSCs的研究中仍需要通过多个表面分子联合标记,并结合自我更新和多向分化潜能的特性对其进行鉴定,这给MSCs的检测和分选带来一定难度,也在一定程度上限制了MSCs的应用。本课题组以人BM-MSCs为免疫原,应用杂交瘤技术制备了四种抗人骨髓间充质干细胞单克隆抗体,分别命名为ZUB1、ZUB4、ZUC3、ZUF10。研究已证实四种单克隆抗体与骨髓来源的8个细胞株(HL-60、NB4、K562、U937、HEL、Jurkat、Raji、KM3)和11种人间充质组织和非间充质组织标本(韧带、肌腱、神经、脂肪、肌肉、血管壁、表皮、肺、肝、小肠、胆囊)均无交叉反应。本实验应
[Abstract]:Bone marrow mesenchymal stem cells ( BM - MSCs ) are another type of bone marrow stem cells which are different from hematopoietic stem cells , and have self - renewal , high proliferation and multi - directional differentiation potential , and can be differentiated into adipocytes , chondrocytes , osteoblasts , astrocytes and neuronal cells under the induction conditions in vivo or in vitro . Recent studies have shown that mesenchymal stem cells have weak immunogenicity and strong immunoregulatory functions , and these biological characteristics have broad application prospects in the fields of tissue engineering , gene therapy , cell transplantation , and the like . In the 1990s , the researchers tried to find the specific surface markers of BM - MSCs , and the monoclonal antibodies related to MSCs were prepared by hybridoma technique , such as sh2 , SH3 , SH4 , STRO - 1 , etc . Four monoclonal antibodies against human bone marrow mesenchymal stem cells were prepared from human BM - MSCs , named ZUB1 , ZUB4 , ZUC3 , ZUF10 . Four monoclonal antibodies were identified as ZUB1 , ZUB4 , ZUC3 , ZUF10 .

【学位授予单位】：浙江大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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