不同应激对小鼠免疫防御功能的影响及其分子免疫学基础

发布时间：2017-12-31 09:24

本文关键词：不同应激对小鼠免疫防御功能的影响及其分子免疫学基础　出处：《大连医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 研究表明应激能够影响动物和人体的免疫功能,机体免疫系统功能的紊乱是感染性疾病、肿瘤等发生或恶化的直接原因,故阐明应激对免疫系统损伤的作用机制具有重要意义。动物模型具有资源相对易获得、操作性强、涉及伦理道德问题相对较少等优点,因此建立理想的应激动物模型是研究得以深入的关键。我们应用束缚应激和热应激建立了两种小鼠应激动物模型,然后使小鼠染菌,通过比较应激状态下小鼠与正常小鼠的死亡率来研究两种应激对小鼠免疫防御功能的影响,并试图从遗传背景角度揭示基因多态性与应激状态下免疫应答强度的关系;通过应激所导致的机体免疫器官损伤、血清中蛋白质的变化及全血中金属元素含量的变化几个方面来揭示应激对免疫防御功能影响的相关机制。方法应用束缚应激和热应激建立了两种小鼠应激动物模型,然后使小鼠染菌,研究这两种应激对小鼠免疫防御功能影响,并通过以下几个方面来研究应激对免疫功能损伤的可能机制。 1.研究应激对小鼠脾脏重量指数的影响,观察接受应激的小鼠脾脏的病理变化,阐述免疫防御功能降低的构效关系。 2.以已经建立的动物模型为研究对象,采用PCR技术检测以MudoEb5和MudoEb7为代表的小鼠H2-Eb位点等位基因的多态性,从遗传多样性的角度揭示基因多态性与应激状态下免疫应答强度的关系。 3.采用SELDI-TOF-MS技术,运用弱阳离子交换芯片(WCX2)对小鼠血清蛋白质指纹图谱进行研究,探索不同类型的应激对免疫功能影响的特点及物质基础。
[Abstract]:Studies have shown that stress can affect the immune function of animals and human beings, and the disorder of immune system is the direct cause of infectious diseases, tumors and so on. Therefore, it is of great significance to clarify the mechanism of stress on immune system damage. Animal models have the advantages of relatively easy access to resources, strong maneuverability, and relatively few ethical and moral problems. Therefore, the establishment of ideal animal models of stress is the key to further study. We used restraint stress and heat stress to establish two kinds of animal models of mouse stress, and then made the mice infected with bacteria. By comparing the mortality of mice under stress and that of normal mice, the effects of two kinds of stress on the immune defense function of mice were studied. From the perspective of genetic background, this paper attempts to reveal the relationship between gene polymorphism and the intensity of immune response under stress. The mechanism of the effect of stress on immune defense function was revealed from several aspects, such as the damage of immune organ, the change of protein in serum and the change of metal element content in whole blood. Method Two kinds of animal models of mouse stress were established by restraint stress and heat stress, and then the mice were infected with bacteria to study the effects of these two stresses on the immune defense function of mice. And through the following aspects to study the possible mechanism of stress damage to immune function. 1. To study the effect of stress on spleen weight index of mice, observe the pathological changes of spleen of mice receiving stress, and elucidate the structure-activity relationship of the decrease of immune defense function. 2. The alleles of H2-Eb locus in mice represented by MudoEb5 and MudoEb7 were detected by PCR technique. From the perspective of genetic diversity, the relationship between genetic polymorphism and the intensity of immune response under stress was revealed. 3. The fingerprint of serum protein in mice was studied by using SELDI-TOF-MS and WCX2 (weak cationic exchange chip). To explore the characteristics and material basis of the effects of different types of stress on immune function.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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