流感病毒核酸疫苗母源性抗体的保护性与干扰性研究

发布时间：2017-12-31 16:22

本文关键词：流感病毒核酸疫苗母源性抗体的保护性与干扰性研究　出处：《湖南师范大学》2005年硕士论文　论文类型：学位论文

【摘要】：本论文共有两部分组成:(1) 流感病毒血凝素(hemagglutinin,HA)、神经氨酸酶(neuraminidase,NA)核酸疫苗诱导的母源性抗体保护子鼠抗致死量流感病毒攻击能力的研究;(2) 在子鼠体内有母源性抗体的情况下,给子鼠接种不同的流感疫苗,寻找一种既能解除母源性抗体干扰作用,又能很好的保护子鼠抵御致死量流感病毒攻击的新型免疫途径。婴幼儿免疫系统不成熟,对接种疫苗存在耐受性、危险性和免疫应答能力低下等问题,因此母源性抗体成为保护婴幼儿早期抵御流感病毒感染的重要方式之一。实验中我们分别用HA、NA、(HA+NA)的混和疫苗免疫6～8周龄的BALB/c雌鼠两次,二免后七天,将雌鼠与未经免疫的BALB/c雄鼠交配,所产子鼠在不同的周龄分别用同型致死量流感病毒A/P R/8/34进行攻击,通过测定子鼠存活率、体内抗体滴度及肺部病毒残余滴度,得出结论:HA DNA接种母体能够保护2周龄幼龄鼠抵御致死量流感病毒的攻击,NA DNA接种母体能够保护2,3,4周龄子鼠抵御致死量流感病毒的攻击,(HA+NA)混合疫苗接种母体不仅能够保护幼龄鼠而且能够保护5,6周龄的成年子鼠抵御致死量流感病毒的攻击。这说明流感病毒HA DNA、NA DNA能用于母体免疫保护婴幼儿抵御致死量流感病毒攻击,两种疫苗联合应用对子代的保护效果更佳。母源性抗体一方面能够保护新生儿和婴幼儿早期抵御病毒和细菌的感染,但另一方面它能在婴幼儿体内持续存在一段时间,抑制婴幼儿疫苗接种后主动免疫应答的建立。因此,寻
[Abstract]:This thesis consists of two parts: (1) influenza virus hemagglutinin neuraminidase (hemagglutinin, HA), (neuraminidase, NA) of DNA vaccine induced by maternal antibodies to protect rats against lethal dose virus attack ability; (2) in the rat in vivo maternal antibody, were inoculated to different influenza vaccine, to find a way to relieve maternal antibody interference, and new way of immune good protection against mouse lethal dose of influenza virus attacks.
The infant immune system is not mature, there is tolerance to vaccination, the problem of danger and the immune response ability is low, so the maternal antibody has become one of the important ways to protect infants against influenza virus infection. In the experiment we used HA NA (HA+NA), respectively, the mixed vaccine 6 to 8 weeks of age BALB/c two female rats, two free after seven days, the female rats with non immunized BALB/c mice mating offspring in different weeks respectively with the same type of a lethal dose of influenza virus A/P R/8/34 attack, through the determination of offspring survival rate, the antibody titer in the lungs and the residual virus titer, concluded: HA DNA inoculation can protect young maternal rats aged 2 weeks against a lethal dose of influenza virus attacks, NA DNA can protect the 2,3,4 maternal vaccination week old offspring rats against lethal dose of influenza viruses (HA+NA), mixed vaccine can not only protect the mother's body The young mice and can protect the 5,6 week old adult offspring against a lethal dose of influenza virus attack. This shows that the influenza virus HA DNA, NA DNA can be used for the maternal immune protection of infants against a lethal dose of influenza virus attacks, two kinds of better protection effect of combined application of vaccine in offspring.
On the one hand, maternal antibodies can protect newborns and infants from viral and bacterial infections, but on the other hand, they can persist in infants for a period of time, and inhibit the establishment of active immune response after vaccination.

【学位授予单位】：湖南师范大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R392

【引证文献】