猴免疫缺陷病毒SIVmac239感染中国恒河猴建立AIDS动物模型的研究

发布时间：2017-12-31 19:20

本文关键词：猴免疫缺陷病毒SIVmac239感染中国恒河猴建立AIDS动物模型的研究　出处：《吉林农业大学》2006年硕士论文　论文类型：学位论文

【摘要】：动物模型是研究许多疾病不可缺少的工具。因灵长类动物在进化关系上与人类最为接近，所以非人灵长类动物已成为研究人类疾病十分重要的实验动物。自从证实HIV是AIDS病因后，即开始寻找和研究有关合适感染HIV的动物模型。经过大量实验筛选，发现非人灵长类动物是较为理想的用于HIV发病机制、药物治疗和疫苗开发的动物模型，现已广泛应用于AIDS的研究领域。建立合适的动物模型已成为国家重大需求的基础问题，，这将有助于我国对AIDS感染和发病机理的研究，推动抗AIDS新药和疫苗的开发，从而有利于AIDS的防制工作。本论文研究中，首先采用分子克隆技术复建SIVmac239和SHIV89.6病毒株。然后用SIVmac239静脉感染2只中国恒河猴，建立AIDS非人灵长类动物模型。攻毒后，定期对体重、外周血CD4~+T细胞数量、PBMC前病毒DNA、抗体、组织器官病理变化进行检测，共监测121天。在感染早期，便可从宿主细胞中检测到前病毒，并分离出具感染性的SIVmac239；同时，外周血CD4~+T淋巴细胞急剧下降，并一直维持在一个较低水平；对血浆抗体的检测结果表明，动物机体产生了针对病毒的特异性体液免疫反应；在感染121天时，肠道、部分淋巴结等组织发生一定程度的病理变化，特别在小肠粘膜部位发生淋巴组织增生。这些结果说明，SIVmac239成功感染2只中国恒河猴，可应用这种模型进行AIDS相关研究工作。
[Abstract]:Animal models are powerful tools for many diseases. Because primate animal in evolutionary relationship with most similar to human, so non-human primate animal experimental animal studies of human disease has become very important. Since that HIV is the cause of AIDS, which began to look for and Research on appropriate animal models of HIV infection after a lot of experiments. Screening, found the non-human primate animal is an ideal animal model for the pathogenesis of HIV, drug therapy and vaccine development, research field and has been widely used in AIDS. To establish a suitable animal model has become the basic problem of the major needs of the country, which will be helpful to our study of AIDS infection and pathogenesis. To promote the development of anti AIDS drugs and vaccines, which is conducive to the prevention and control of AIDS.
In this paper, firstly by molecular cloning technique and rehabilitation SIVmac239 SHIV89.6 strains. Then using SIVmac239 2 China intravenous infection of monkeys in Ganges RIver, the establishment of non-human primate animal models of AIDS. When challenged on a regular basis weight, the number of peripheral blood CD4~+T cells, PBMC virus DNA antibody, the histopathological changes of detection, were monitored for 121 days. In the early stage of infection, the virus can be detected from the host cells, and isolated infection of SIVmac239; at the same time, peripheral blood CD4~+T lymphocytes decreased sharply, and has remained at a low level; the detection of plasma antibodies showed that the animal body to produce specific humoral immune response to virus infection; intestinal in 121 days, and the pathological changes in some lymph nodes tissue to a certain extent, especially in parts of intestinal mucosal lymphoid hyperplasia. These results suggest that SIV Mac239 has successfully infected 2 Chinese Ganges RIver monkeys. This model can be used for AIDS related research.

【学位授予单位】：吉林农业大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R-332

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