细胞核DNA倍体分析在细胞涂片中的应用

发布时间：2018-01-01 16:06

本文关键词：细胞核DNA倍体分析在细胞涂片中的应用　出处：《暨南大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：第一部分：探索一种快速的Feulgen染色方法，并尝试其在细胞核DNA含量定量分析中的应用。第二部分：评估TIGER细胞图像分析仪测量细胞图像光度的线性。第三部分：(1)比较细胞核DNA倍体分析与常规细胞学检查这两种方法对宫颈癌及癌前病变筛查的灵敏度。 (2) 探索用细胞核DNA倍体分析进行宫颈癌及癌前病变的普查，为提高普查的工作效率和准确性找到一种更加有效的方法。方法：第一部分：选取十只健康小白鼠的肝细胞涂片，每只小白鼠的肝细胞涂片分成三组，采用快速Feulgen染色法、改良Feulgen染色法、传统Feulgen染色法对这三组分别染色，用TIGER细胞图像分析仪测量肝细胞涂片内单个完整肝细胞核的DNA含量，比较各组染色效果和肝细胞核DNA含量的测量结果。第二部分：选取十只健康成年小白鼠的睾丸组织，制成细胞悬液，涂片，快速Feulgen染色，TIGER细胞图像分析仪测量涂片内三种生精细胞单个完整细胞核的DNA含量并比较它们之间的比值，分析其平均光密度(AOD)的最大、最小值范围。第三部分：对100例妇女用宫颈刷取材，液基薄层制片。巴氏染色做常规细胞学检查，Feulgen染色做细胞核DNA倍体分析。经常规细胞学检查和细胞核DNA倍体分析，对可疑的病例进行病理组织学检查。结果：第一部分：(1)应用快速Feulgen染色法，获得了满意的效果，，细胞核染色深，
[Abstract]:Objective: Part one: to explore a rapid method of Feulgen staining and its application in quantitative analysis of nuclear DNA content. Part two: evaluate the linearity of cell image luminosity measured by TIGER cell image analyzer. Part 3: 1) the sensitivity of nuclear DNA ploidy analysis and conventional cytology for screening cervical cancer and precancerous lesions was compared. (2) to explore a more effective method for the screening of cervical cancer and precancerous lesions by nuclear DNA ploidy analysis in order to improve the efficiency and accuracy of the census. Methods: The first part: the hepatocyte smears of ten healthy mice were divided into three groups. The rapid Feulgen staining method and the modified Feulgen staining method were used. The three groups were stained with traditional Feulgen staining method. The DNA content of a single intact liver nucleus was measured by TIGER cell image analyzer. The results of staining and DNA content were compared. The second part: the testicular tissue of ten healthy adult mice was selected and made into cell suspension smear and rapid Feulgen staining. TIGER cell image analyzer was used to measure the DNA content in the single intact nucleus of three spermatogenic cells in the smear and to compare the ratio between them, and to analyze the maximum average optical density of the three spermatogenic cells. Minimum range. The third part: 100 cases of women with cervical brushes, liquid-based thin film, pasteurized staining for routine cytological examination. The nuclear DNA ploidy was analyzed by Feulgen staining. The pathological examination of suspicious cases was made by routine cytological examination and nuclear DNA ploidy analysis. Results: Part 1: 1) the rapid Feulgen staining method was used to obtain satisfactory results.
【学位授予单位】：暨南大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R329

【相似文献】