通过诱导P物质的表达可促进HIV病毒对人类T淋巴细胞的感染—吗啡戒断的体外模型研究

发布时间：2018-01-02 00:11

本文关键词：通过诱导P物质的表达可促进HIV病毒对人类T淋巴细胞的感染—吗啡戒断的体外模型研究　出处：《华中师范大学》2007年硕士论文　论文类型：学位论文

更多相关文章：吗啡 HIV P物质 戒断 CP-96 345 淋巴细胞

【摘要】： 鸦片类毒品的停用在鸦片类毒品的滥用过程中是一个至关紧要和容易发生的现象，它对于吸毒人员免疫系统的功能有着很大的负面影响。在此项课题中，我们研究了“突然停药”和“彻底断药”是否能促进HIV病毒对人类T淋巴细胞的感染。研究结果显示“突然停药”和“彻底断药”都能促进HIV病毒对人类外周血原代淋巴细胞和T淋巴瘤细胞系(Jurkat细胞系及CEMX174细胞系)的感染。此外，“突然停药”和“彻底断药”均可诱导HIV病毒在潜伏感染HIV的人类T淋巴瘤细胞系(J1.1细胞系)中的复制。这些“突然停药”和“彻底断药”对HIV病毒感染及复制的促进效果，在人类外周血原代淋巴细胞和T淋巴瘤细胞系上表现出与神经肽P物质的诱导表达相关联。P物质受体的拮抗剂，CP-96，345，在T细胞上不仅能阻断“突然停药”和“彻底断约”对P物质的诱导表达，，而且还能阻断“突然停药”和“彻底断药”对HIV病毒感染及复制的促进影响。此项研究成果对于支持“吗啡在促进HIV病毒对免疫细胞的感染及复制过程起着重要作用”这一学术观点提供了重要的细胞Qg相关机制。
[Abstract]:The discontinuation of opiates is a crucial and easy phenomenon in the process of opiate abuse, which has a great negative impact on the immune system of drug addicts. We studied whether "sudden withdrawal" and "complete withdrawal" promoted the infection of human T lymphocytes by HIV virus. The results showed that "sudden withdrawal" and "complete withdrawal" of drugs. Both can promote the effect of HIV virus on human peripheral blood primary lymphocytes and T lymphoma cell lines (. Jurkat cell line and CEMX174 cell line) infection. Both "sudden withdrawal" and "complete withdrawal" can induce HIV virus in human T lymphoma cell line J1.1, which is potentially infected with HIV). The effects of these "sudden withdrawal" and "complete withdrawal" on HIV infection and replication. In human peripheral blood primary lymphocytes and T lymphoma cell lines, it was shown that the antagonist of substance P receptor was CP-96N345, which was associated with the induced expression of neuropeptide substance P. On T cells, the induced expression of substance P was not only blocked by "sudden withdrawal" and "complete withdrawal". It can also block the effect of "sudden withdrawal" and "complete withdrawal" on the infection and replication of HIV virus. The academic view that morphine plays an important role in promoting the infection and replication of HIV virus to immune cells provides an important mechanism of cell QG correlation.
【学位授予单位】：华中师范大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R373

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