内吗啡肽在大鼠脊髓背角浅层调控外周伤害性信息传递的机制

发布时间：2018-01-02 01:15

本文关键词：内吗啡肽在大鼠脊髓背角浅层调控外周伤害性信息传递的机制　出处：《第四军医大学》2005年硕士论文　论文类型：学位论文

【摘要】：内吗啡肽-1(EM-1)和内吗啡肽-2(EM-2)是μ型阿片受体(MOR)的内源性配体。MOR激活后能够抑制腺苷酸环化酶,开启胶状质(SG)神经元上的钾通道和抑制背根节神经元的钙电流。EMs和MOR在脊髓背角浅层内均有大量的分布。脊髓背角浅层(Ⅰ、Ⅱ层)是接受外周伤害性信息的初级门户,伤害性信息在此中继、整合后,由投射神经元向上位脑结构传递。P物质(SP)是初级传入纤维传递伤害性信息的重要神经活性物质,脊髓背角浅层内有密集的SP阳性纤维和终末,并且多数SP阳性纤维和终末也同时表达MOR。大量的实验证据表明EMs在脊髓水平发挥明确的镇痛作用,但是EMs在脊髓水平参与伤害性信息传递的细胞和分子机制仍不清楚。故本研究应用脊髓薄片膜片钳全细胞记录技术观察了EMs对成年大鼠SG内兴奋性和抑制性突触传递的影响以及EMs在脊髓背角浅层内对初级传入终末释放SP的影响,以期进一步阐明EMs在脊髓水平镇痛作用的机制。结果表明:(1) EMs抑制SG内兴奋性和抑制性突触传递。EM-1(1 μmol/L)和EM-2(1 μmol/L)都能够显著抑制微小兴奋性突触后电流(mEPSCs)和微小抑制性突触后电流(mIPSCs)的频率而不改
[Abstract]:Endomorphine -1 (EM-1) and endomorphine -2 (EM-2) is opioid receptor (MOR) of the endogenous ligand.MOR can inhibit the activation of adenylate cyclase (SG), open the substantia gelatinosa neurons and inhibition of potassium channels in dorsal root ganglion neurons of calcium current.EMs and the distribution of MOR in spinal cord dorsal there are a lot of angle in the superficial layers. The superficial layers of the spinal dorsal horn (I, II layer) is the primary portal for peripheral nociceptive information, nociceptive information in the relay, after integration, by projecting neurons to the brain structure transfer of substance.P (SP) is an important neurotransmitter of primary afferent fibers transmit nociceptive signal the superficial layers of the spinal dorsal horn in the dense SP positive fibers and terminals, and the majority of SP positive fibers and terminals also express MOR. substantial experimental evidence indicates that EMs plays a significant analgesic effect at the spinal level, but the level of EMs in the spinal cord injury involved in the information transmission of fine The cellular and molecular mechanisms are still unclear. So the research and application of spinal cord slice patch clamp whole cell recording technique to observe the EMs of adult rat SG in excitatory and inhibitory synaptic transmission and the effect of EMs in the spinal dorsal horn effect on the release of SP from primary afferent terminals in the superficial layers, in order to further elucidate the mechanism of EMs in spinal cord the level of analgesia.
The results showed that: (1) EMs inhibited the excitatory and inhibitory synaptic transmission in SG,.EM-1 (1 mol/L) and EM-2 (1 mol/L) could significantly inhibit the frequency of small excitatory postsynaptic current (mEPSCs) and tiny inhibitory postsynaptic current (mIPSCs).

【学位授予单位】：第四军医大学
【学位级别】：硕士
【学位授予年份】：2005
【分类号】：R33

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