水痘—带状疱疹病毒糖蛋白E DNA疫苗的免疫原性研究

发布时间：2018-01-03 06:18

本文关键词：水痘—带状疱疹病毒糖蛋白E DNA疫苗的免疫原性研究　出处：《四川大学》2005年博士论文　论文类型：学位论文

【摘要】：水痘-带状疱疹病毒(VZV)为DNA双链病毒,长度约为125000个碱基对。现已发现VZV基因组有69个开放读码框(ORFs),至少可以编码6种糖蛋白,相对分子量从20×10~3到118×10~3,分别为gE、gB、gH、gI、gC和gL。其中糖蛋白E(gE)是第68个开放读码框的编码产物,初期翻译产物的相对分子量为73×10~3,成熟的糖基化产物为88×10~3～98×10~3。糖蛋白E是病毒感染细胞表面最丰富的糖蛋白,能够诱导中和抗体和T辅助细胞反应,并且含有被CD4~+和CD8~+细胞识别的抗原决定簇。水痘-带状疱疹病毒可引起水痘与带状疱疹两种常见疾病,主要感染新生儿、成人和免疫缺陷的患者,后遗神经痛是常见的并发症。VZV减毒活疫苗(Oka株)已在世界范围内广泛应用多年。虽然该疫苗可以有效的预防严重的水痘,但是该疫苗仍存在潜伏性和体内复活等问题,所以国外已有学者开展了关于VZV新型疫苗的研究。其中所采用的DNA免疫技术在试验阶段已经能够预防病毒、细菌和寄生虫感染,比传统的以蛋白为基础的疫苗预防有更大的优势。本研究选择水痘-带状疱疹病毒DNA疫苗这一研究领域,构建了水痘-带状疱疹病毒糖蛋白E DNA疫苗,并用核酸免疫技术评价其免疫原性。
[Abstract]:Varicella zoster virus (VZV) is a double stranded DNA virus, a length of about 125000 base pairs. It has been found that the VZV genome has 69 open reading frames (ORFs), at least 6 encoding glycoprotein, relative molecular weight from 20 * 10~3 to 118 * 10~3, respectively gE, gB, gH. GI, gC and gL. E (gE) is a glycoprotein encoding product of sixty-eighth open reading frames, the initial relative molecular weight of the translation product is 73 * 10~3, glycosylation product maturity is 88 * 10~3 to 98 * 10~3. E protein is the most abundant cell surface glycoprotein of the virus infection can induce neutralizing antibody and T helper cell responses, and contains CD4~+ and identification of CD8~+ cell epitopes.
Varicella zoster virus can cause the two common diseases of varicella and herpes zoster infection, mainly neonatal, adult and immunodeficiency patients with postherpetic neuralgia is a common complication of.VZV live attenuated vaccine (Oka strain) has been widely used in the world for many years. Although the vaccine can effectively prevent severe varicella, but the there are still latent vaccine in vivo and raised other issues, so foreign scholars have done research on new vaccines against VZV. DNA immune technology which had been adopted to prevent the virus in the test phase, bacteria and parasite infection, have more advantages than conventional protein based vaccine prevention.
In the research area of varicella zoster virus DNA vaccine, we constructed varicella zoster virus glycoprotein E DNA vaccine and evaluated its immunogenicity by nucleic acid immunoassay.

【学位授予单位】：四川大学
【学位级别】：博士
【学位授予年份】：2005
【分类号】：R392.1

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