心理应激对小鼠行为和免疫功能的影响

发布时间：2018-01-03 11:12

本文关键词：心理应激对小鼠行为和免疫功能的影响　出处：《山西医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 目的:通过观察慢性心理应激对小鼠情绪行为和认知能力的影响以及免疫器官的变化、血清中IFN-γ和IL-4水平和Th1和Th2细胞的失衡状况,探讨慢性心理应激对心理行为和免疫功能的影响及可能的机制,为亚健康人群开展有效的防护和干预提供理论依据。方法: BALB/c小鼠40只,随机分为对照组、5天应激组、10天应激组、15天束缚应激组,每组10只。采用自制的束缚筒对小鼠进行连续束缚的方法建立慢性心理应激模型,应激结束后采用旷场实验和Morris水迷宫实验观察小鼠应激状态下的行为变化;处死,测量各组小鼠胸腺、脾脏指数及脾和肠系膜淋巴结淋巴细胞数量,并以ELISA法检测其血清IFN-γ和IL-4的水平及IL-4/IFN-γ比值的变化。结果:心理应激使小鼠在旷场实验中的潜伏期显著延长(P0.01),探究活动减少(P0.05和P0.01),粪便次数增多(P0.05和P0.01),Morris水迷宫中各应激组小鼠寻找平台的潜伏期较对照组延长(P0.05和P0.01),在空间探索实验中,应激组小鼠在平台所在象限的游泳时间比例均小于对照组。提示慢性心理应激可导致小鼠焦虑、抑郁和认知功能的障碍。应激组小鼠的体重始终受到抑制低于对照组,差异显著(P0.05和P0.01)。各应激组小鼠的脾脏指数及脾和肠系膜淋巴结淋巴细胞数量受到明显抑制,5天应激组脾脏指数明显降低(P0.05),10和15天应激组显著低于对照组(P0.01),而胸腺指数虽低于对照但无显著意义。IFN-γ、IL-4/IFN-γ比值明显下降,与正常对照比较(P0.05和P0.01),而IL-4水平无显著性差异,提示心理应激抑制小鼠的体重和免疫功能,导致Th1/Th2平衡失调,向Th2细胞漂移,并且随着应激时间的延长变化越明显。脾脏指数及脾和肠系膜淋巴结淋巴细胞、IFN-γ与穿格、修饰之间呈显著正相关,与中央格停留时间和粪便呈负相关。提示应激所致的情绪行为和认知障碍可能与免疫功能的抑制有关。结论:慢性心理应激可以引起机体的情绪行为和认知功能的改变,主要表现为情绪的焦虑、抑郁和学习记忆、适应、反应等认知能力的下降;慢性心理应激可以影响体重和免疫功能,导致机体的体重的改变和免疫力的下降,从而引起体质衰弱、病感或疾病;心理应激引起情绪行为和认知功能的改变与免疫功能相关。因此,心理应激引起机体的心理行为和免疫功能的改变可能是亚健康状态的主要原因。
[Abstract]:Objective: to observe the effects of chronic psychological stress on emotional behavior and cognitive ability, the changes of immune organs, the levels of IFN- 纬 and IL-4 in serum and the imbalance of Th1 and Th2 cells in mice. To explore the influence of chronic psychological stress on psychological behavior and immune function and its possible mechanism, to provide theoretical basis for effective protection and intervention of sub-healthy population. Methods: forty BALB/c mice were randomly divided into 5 days stress group and 15 days restraint stress group. The chronic psychological stress model was established with 10 mice in each group. After stress, open field test and Morris water maze test were used to observe the behavior of mice under stress. The thymus, spleen index and lymphocyte number of spleen and mesenteric lymph nodes were measured. The levels of serum IFN- 纬 and IL-4 and the ratio of IL-4 / IFN- 纬 were measured by ELISA method. Results: psychological stress significantly prolonged the incubation period of mice in open field test, and decreased the activity of inquiry (P0.05 and P0.01). Fecal frequency increased P0.05 and P0.01 Morris water labyrinth of mice in the search for platform latency prolonged compared with the control group (P0.05 and P0.01). In the space exploration experiment, the proportion of swimming time in the platform quadrant in the stress group was lower than that in the control group, suggesting that chronic psychological stress could lead to anxiety in the mice. Depression and cognitive impairment. The body weight of the stress group was always inhibited as compared with the control group. The spleen index and the number of lymphocytes in spleen and mesenteric lymph nodes were significantly inhibited in each stress group. The spleen index of 5-day stress group was significantly lower than that of control group on day 10 and 15, while the thymus index was lower than that of control group. The ratio of IL-4 / IFN- 纬 was significantly lower than that of normal controls (P0.05 and P0.01), but there was no significant difference in IL-4 level. These results suggest that psychological stress inhibits the body weight and immune function of mice and results in imbalance of Th1/Th2 balance and drift to Th2 cells. The spleen index and lymphocyte IFN- 纬 of spleen and mesenteric lymph node were positively correlated with translattice. There was a negative correlation between the residence time of central lattice and feces, suggesting that the emotional behavior and cognitive impairment induced by stress might be related to the inhibition of immune function. Conclusion: chronic psychological stress can cause the changes of emotional behavior and cognitive function, mainly manifested as emotional anxiety, depression, learning and memory, adaptation, response and other cognitive ability decline. Chronic psychological stress can affect body weight and immune function, leading to body weight change and immune decline, resulting in physical weakness, disease or disease; The changes of emotional behavior and cognitive function caused by psychological stress are related to immune function, therefore, the changes of psychological behavior and immune function may be the main causes of sub-health state.
【学位授予单位】：山西医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R395

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