脑缺血小鼠大脑皮层CD4、CD8免疫反应观察及脾T淋巴细胞亚群变化

发布时间：2018-01-03 14:34

本文关键词：脑缺血小鼠大脑皮层CD4、CD8免疫反应观察及脾T淋巴细胞亚群变化　出处：《大连医科大学》2006年硕士论文　论文类型：学位论文

【摘要】： 背景和目的: 免疫机制与中枢神经系统损伤、肿瘤、缺血以及退变性疾病密切相关。急性缺血性脑卒中是中枢神经系统的急性损伤性病变,有研究表明,免疫机制参与了缺血性脑损伤的发生发展过程。脑缺血时,激活的小胶质细胞,浸润的外周血多形核白细胞、中性粒细胞和单核细胞等炎性细胞介入脑缺血性损伤的病理过程外,机体的免疫细胞水平也有变化。当T淋巴细胞亚群的数量和功能异常时,机体可产生免疫紊乱而导致机体发病。T细胞亚群与脑缺血的联系近年来倍受重视。本实验研究的主要目的在于观察脑缺血后脑组织淋巴细胞浸润情况及脾脏T细胞亚群的变化,探讨淋巴细胞及其亚群在缺血性脑损伤中的作用。方法: 研究对象为健康成年雄性Balb/c小鼠,光化学法建立小鼠局灶性脑缺血模型,分为对照组、单纯光敏剂组和脑缺血组,各组又分为脑缺血后6h、1d、3d和7d四个亚组。在处死前半小时,无菌条件下取出脾脏,制备单个核细胞悬液,用于流式细胞仪检测脾脏CD4~+、CD8~+T淋巴细胞百分率及CD4~+/CD8~+的比值。在相应时间点处死动物,通过Nissl染色观察受损脑区皮层的形态学变化,免疫组织化学染色检测皮层CD4和CD8阳性细胞的反应。结果: Nissl染色显示:对照组和单纯光敏剂组皮层无损伤,细胞分布和形态均正常,两组之间无差异。与对照组相比,缺血组6h就出现损伤区,1d和3d可见梗死区与周围正常脑组织有一明显的分界线,缺血周
[Abstract]:Background and purpose: Immune mechanism is closely related to central nervous system injury, tumor, ischemia and degenerative diseases. Acute ischemic stroke is an acute lesion of the central nervous system. Immune mechanism is involved in the occurrence and development of ischemic brain injury. During cerebral ischemia, activated microglia and infiltrated peripheral blood polymorphonuclear leukocytes. In addition to the involvement of inflammatory cells such as neutrophils and monocytes in the pathological process of ischemic brain injury, the level of immune cells in the body also changes, when the number and function of T lymphocyte subsets are abnormal. The relationship between the pathogenesis of T cell subsets and cerebral ischemia has been paid more and more attention in recent years. The main purpose of this experiment is to observe the infiltration of lymphocytes in brain tissue and spleen T after cerebral ischemia. Changes in cell subsets. To investigate the role of lymphocyte and its subsets in ischemic brain injury. Methods: The model of focal cerebral ischemia in healthy adult male Balb/c mice was established by photochemical method. The mice were divided into three groups: control group, simple Guang Min group and cerebral ischemia group. Each group was divided into 6 hours after cerebral ischemia. The spleen was taken out half an hour before the death and the mononuclear cell suspension was prepared for the detection of CD4 ~ by flow cytometry. The percentage of CD8T lymphocytes and the ratio of CD4- / CD8. the animals were killed at the corresponding time points, and the morphological changes of the damaged cerebral cortex were observed by Nissl staining. Immunohistochemical staining was used to detect the reaction of CD4 and CD8 positive cells in cortex. Results: Nissl staining showed that there was no damage to cortex, cell distribution and morphology were normal in the control group and Guang Min group, and there was no difference between the two groups. Compared with the control group, the injured area appeared at 6 hours in the ischemic group. On the 1st and 3rd days, there was a clear boundary between the infarcted area and the surrounding normal brain tissue, and the ischemic week.
【学位授予单位】：大连医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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