H-Ras介导细胞转化的蛋白质组学分析

发布时间：2018-01-03 18:41

本文关键词：H-Ras介导细胞转化的蛋白质组学分析　出处：《中国人民解放军军事医学科学院》2007年博士论文　论文类型：学位论文

更多相关文章： Ras 细胞转化 蛋白质组学 gankyrin

【摘要】： Ras是ras基因编码的小GTP结合蛋白，在人肿瘤细胞中的基因突变率大约为30％。它作为细胞内重要的功能性蛋白质之一，Ras自从发现至今20多年的时间里，国内外的众多实验室对其功能和机制进行了广泛的研究和探讨。虽然Ras在细胞转化和肿瘤发生过程中起着很重要的作用，但是其具体的作用机制还不十分清楚。在本研究工作中，我们通过蛋白质组学手段对转染了激活型Ras(H-RasV12)的NIH3T3细胞进行了全景式的蛋白质组分析，共发现了44个Ras转化相关的蛋白质，其中28个蛋白质以前从未见与Ras转化相关的报道。进而我们用RT-PCR发现鉴定出的蛋白质中大约有44％在mRNA水平就发生了变化。在这些差异蛋白质中，我们对其中一个上调蛋白质(gankyrin)做了进一步的研究，结果表明gankyrin在Ras转化过程中扮演了重要角色，是细胞转化过程中潜在的靶蛋白，但要阐明gankyrin参与细胞转化的详细机制，，仍需要进一步深入分析。
[Abstract]:Ras is a small GTP binding protein encoded by ras gene and its mutation rate in human tumor cells is about 30. It is one of the important functional proteins in cells. It has been more than 20 years since Ras was discovered. Many laboratories at home and abroad have extensively studied its function and mechanism, although Ras plays an important role in the process of cell transformation and tumorigenesis. However, its specific mechanism is not very clear. In this study. We performed a panoramic proteome analysis of NIH3T3 cells transfected with activated RasH-RasV12 by proteomics. A total of 44 proteins related to Ras transformation were found. 28 of these proteins have never been reported to have been associated with Ras transformation before. About 44% of the proteins identified by RT-PCR have changed at the mRNA level. In different proteins. We further studied one of the up-regulated protein, gankyrin. the results showed that gankyrin plays an important role in the process of Ras transformation. It is a potential target protein in the process of cell transformation, but to elucidate the detailed mechanism of gankyrin involved in cell transformation, further analysis is needed.
【学位授予单位】：中国人民解放军军事医学科学院
【学位级别】：博士
【学位授予年份】：2007
【分类号】：R341

【共引文献】