微导管法建立大脑中动脉闭塞性兔脑缺血—再灌注模型

发布时间：2018-01-04 18:04

本文关键词：微导管法建立大脑中动脉闭塞性兔脑缺血—再灌注模型　出处：《延边大学》2007年硕士论文　论文类型：学位论文

【摘要】： 目的探讨建立稳定性、可靠性、可重复性强的动物脑缺血模型的方法。方法日本大耳白兔50只，雌雄不限，随机分为A，B，C三组。其中A组10只，为假手术对照组：B组20只，为微导管栓塞组：C组20只，为线栓组。各组动物麻醉后，暴露一侧CCA、ECA、ICA，A组只暴露动脉不予处理，B组以直径为0.50mm的硬膜外导管经CCA、ICA置入MCA，C组以相同直径的线栓经相同路径置入MCA。MCAO判定方法为，B组采用经特殊拉伸制作的硬膜外导管接医用压力换能器及测压装置用以监测动脉压力波形，，以导管插入达预定深度且动脉压力波形消失作为MCA被成功阻闭的判定标准：C组则按文献根据线栓置入长度作为判定标准。两组动物均于MCAO一小时后恢复再灌注，并于24小时后观察神经学行为改变情况，依照PHILIP评分法进行评分后，(?)头取脑。行TTC及HE染色评价模型效果。结果：B、C两组模型经MCAO后24小时行PHILIP评分及TTC染色及HE染色电镜观察均证实梗死灶存在。成功率：B组为75％，C组为65％。两组比较无显著差异(P＞0.05)。结论：微导管法以压力换能器监测动脉波形作为准确判定MCAO成功标准，效果直观可靠易于操作，是一种理想的制作兔脑缺血-再灌注模型的方法。
[Abstract]:Objective to explore the method of establishing a stable, reliable and reproducible animal model of cerebral ischemia.
Methods 50 Japanese white rabbits, male or female, were randomly divided into A, B, C three groups, 10 rats in A group, sham operation control group: 20 rats in group B, micro catheter embolization group: 20 rats in group C, as the suture group. Each animal after anesthesia, the exposed side of CCA ECA, ICA, A, was only exposed to artery treatment, B group with 0.50mm diameter of the epidural catheter placement of ICA by CCA, MCA, C group with the same diameter of thread through the same path determination method for the placement of MCA.MCAO, B group was treated by epidural catheter with special drawing production and medical pressure transducer pressure measuring device for monitoring the arterial pressure waveform, the catheter reaches a predetermined depth and arterial pressure waveform disappeared as MCA was successfully closed the criteria of resistance: C group according to the literature according to the length of suture placement as criteria. Two animal groups were in the MCAO one hour after reperfusion injury, and after 24 hours of observation the behavior changes of God According to the situation, PHILIP score were used, (?) from the brain. Head TTC and HE staining effect evaluation model. Results: B, C two models by MCAO 24 hours after the PHILIP score and TTC staining and HE staining electron microscopy showed focal infarction. The success rate of B group was 75%. The C group is 65%. between the two groups had no significant difference (P > 0.05). Conclusion: the micro catheter pressure transducer to accurately determine MCAO monitoring the arterial waveform as the standard of success, the effect is direct and reliable and easy to operate, is a kind of ideal of rabbit cerebral ischemia reperfusion model.

【学位授予单位】：延边大学
【学位级别】：硕士
【学位授予年份】：2007
【分类号】：R-332

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