SDF-1α促进原代培养大鼠星形胶质细胞增殖的作用

发布时间：2018-01-05 14:39

本文关键词：SDF-1α促进原代培养大鼠星形胶质细胞增殖的作用　出处：《中国药理学通报》2014年09期 　论文类型：期刊论文

【摘要】：目的研究间质细胞来源因子-1α(stromal cell-derived factor-1α,SDF-1α)对原代培养大鼠星形胶质细胞增殖的作用及其可能机制。方法以不同浓度的重组人SDF-1α作用于星形胶质细胞后,用细胞计数法和溴化去氧尿苷参入法检测细胞增殖;钙离子荧光探针检测细胞内钙离子浓度;Western blot法检测细胞外调节蛋白激酶(extracellular regulated protein kinase,ERK)1/2磷酸化水平;流式细胞仪检测细胞周期;定量RT-PCR法检测Cyclin A2和Cyclin B1的mRNA表达。结果不同浓度的SDF-1α(10-40 nmol·L-1)作用48 h可明显地促进星形胶质细胞的增殖。SDF-1α(20 nmol·L-1)处理可增加细胞内钙离子浓度,提高ERK1/2磷酸化水平;而且可促使细胞由G0期进入DNA合成期(S期)和有丝分裂期(M期),上调Cyclin A2和Cyclin B1的mRNA表达。结论 SDF-1α可明显诱导离体培养大鼠星形胶质细胞增殖,其机制可能是促进钙离子内流,磷酸化ERK1/2,上调Cyclin表达而促进细胞进入DNA合成期和分裂期。
[Abstract]:Objective to study the mesenchymal cells derived factor -1 alpha (stromal cell-derived factor-1 SDF-1 alpha, alpha) proliferation of cultured rat astrocytes of primary and its possible mechanism. Methods the effects of different concentrations of recombinant human SDF-1 alpha in astrocytes after using cell counting and bromodeoxyuridine incorporation assay. Proliferation; calcium fluorescence probe to detect the intracellular calcium concentration of Western cells was determined by blot method; external regulated protein kinase (extracellular regulated protein kinase, ERK) 1/2 phosphorylation; flow cytometry; quantitative expression of RT-PCR A2 and Cyclin Cyclin detected by B1 mRNA. The different concentrations of SDF-1 (alpha 10-40 nmol - L-1) for 48 h can obviously promote the proliferation of astrocytes.SDF-1 alpha (20 nmol L-1) can increase the intracellular calcium concentration increased ERK1/2 phosphorylation level and can promote; The cells from G0 phase to DNA synthesis phase (S phase) and mitotic phase (M phase), Cyclin A2 and Cyclin B1 expression of mRNA. Conclusion SDF-1 alpha can induce cultured rat astrocytes proliferation and its possible mechanism is to promote calcium influx, phosphorylation of ERK1/2. Cyclin expression to induce cells into DNA synthesis and mitosis.

【作者单位】：江苏大学基础医学与医学技术学院药理学系;
【基金】：国家自然科学基金资助项目(No 81300059) 江苏大学高级人才基金项目(No 08JDG005,11JDG092)
【分类号】：R329.2
【正文快照】： R392.11于星形胶质细胞后,用细胞计数法和溴化去氧尿苷参入法检测细胞增殖;钙离子荧光探针检测细胞内钙离子浓度;West-ern blot法检测细胞外调节蛋白激酶(extracellular regulatedprotein kinase,ERK)1/2磷酸化水平;流式细胞仪检测细胞周期;定量RT-PCR法检测Cyclin A2和Cyclin

【共引文献】