氨基葡萄糖及壳寡糖对小鼠免疫功能的影响

发布时间：2018-01-08 11:31

本文关键词：氨基葡萄糖及壳寡糖对小鼠免疫功能的影响　出处：《中国海洋大学》2006年硕士论文　论文类型：学位论文

【摘要】：免疫系统是人体抵抗疾病，特别是抵抗感染性疾病的完整的组织系统，它由免疫组织、免疫器官、免疫细胞以及免疫分子四部分组成。各层次不同类型的免疫组织和细胞在体内发挥不同的功能，通过淋巴细胞再循环和各种免疫分子将各部分的功能协调统一起来。各种免疫细胞能及时清除自身体内的一些异常细胞和代谢物，消除其对机体正常生理功能的影响，维持自身内环境的稳定性。随着现代医学、细胞生物学及分子生物学的发展，人们认识到许多疾病的发生、人体衰老及老年人的多发病都与免疫系统的紊乱有密切关系。研究开发具有促进机体免疫力的功能性食品、药品，，越来越受到研究者的关注。壳聚糖及其衍生物具有广泛的生物学功能，在抗菌、增强机体免疫、伤口愈合、减肥、降血脂及生物材料中具有重要的应用价值。壳寡糖(chito-oligosaccharide，COS)及其单糖衍生物—氨基葡萄糖(glucosamine，GlcNH_2)在体内由于具有易被吸收、转化和利用等特点，较壳聚糖能够发挥更重要的生物学功能，有关COS和GlcNH_2增强免疫功能方面的研究，除壳寡糖有少量文章报道外，GlcNH_2在国内外尚未见详细报道。本研究利用酶水解法制备、纯化了COS和GlcNH_2，并对其各项理化性质指标进行测定。通过体内、体外实验方法研究了COS和GlcNH_2对免疫功能的影响。实验以昆明种小鼠为研究对象，用体外实验方法测定COS和GlcNH_2对体外培养的小鼠脾淋巴细胞增殖能力的影响以及对腹腔巨噬细胞(peritoneal macrophage，PMΦ)增殖能力、吞噬中性红能力、分泌细胞因子(NO和IL-1)功能的影响。将细胞分别与10、100、500、1000μg／ml GlcNH_2进行共培养，测定其对细胞增殖能力、PMΦ吞噬中性红能力、PMΦ分泌NO和IL-1能力的影响。为研究COS和GlcNH_2对体内非特异性免疫功能和体液免疫、细胞免疫的影响，将实验动物进行分组，实验组每天分别灌服不同剂量的COS和GlcNH_2·HCl溶液(125、250、500 mg／kg bw)，连续20天，然后对免疫功能指标进行测定，主要包括单核巨噬细胞吞噬功能(碳粒廓清法)，绵羊红细胞所致小鼠迟发型超敏反应(足跖增厚法)，抗体生成能力(半数溶血值法)，以及小鼠免疫器官指数(脾脏指数和胸腺指数)。
[Abstract]:The immune system is a complete tissue system in which the body resists diseases, especially infectious diseases. It consists of immune tissues, immune organs. Immune cells and immune molecules are composed of four parts. Different types of immune tissues and cells play different functions in the body. Through lymphocyte recirculation and various immune molecules to coordinate the functions of each part. All kinds of immune cells can remove some abnormal cells and metabolites in their bodies in time. With the development of modern medicine, cell biology and molecular biology, many diseases have been recognized. Human aging and the frequent incidence of the elderly are closely related to the disorder of the immune system. The research and development of functional foods and drugs that promote the immunity of the body have been paid more and more attention by researchers. Chitosan and its derivatives have a wide range of biological functions in antibacterial, enhance body immunity, wound healing, weight loss. Chitosan oligosaccharide has important application value in reducing blood lipid and biomaterials. Cos) and its monosaccharide derivative, glucosamine glucosamine, GlcNH2) are easily absorbed, transformed and utilized in vivo. Chitosan can play a more important biological function than chitosan. Studies on the immune function of COS and GlcNH_2 have been reported in a few articles except for chitosan and oligosaccharide. GlcNH_2 has not been reported in detail at home and abroad. In this study, COS and GlcNH2 were purified by enzymatic hydrolysis, and their physicochemical properties were determined. The effects of COS and GlcNH_2 on immune function of Kunming mice were studied in vitro. The effects of COS and GlcNH_2 on the proliferation of murine splenic lymphocytes in vitro and peritoneal macrophages were determined by in vitro method. Peritoneal macrophage. The effects of PM 桅) on proliferation, phagocytosis of neutral red and secretion of cytokines (no and IL-1) were compared with those of 10,100,500 cells. 1 000 渭 g / ml GlcNH_2 was co-cultured to determine its ability of cell proliferation and phagocytic ability of PM 桅 to neutral red. In order to study the effects of COS and GlcNH_2 on nonspecific immune function, humoral immunity and cellular immunity in vivo, experimental animals were divided into groups. The experimental group was administered with different doses of COS and GlcNH_2 路HCl solution for 20 days. Then the immune function indexes were measured, including mononuclear macrophage phagocytosis (carbon particle clearance method), sheep erythrocyte induced delayed type hypersensitivity (foot-metatarsal thickening method). Antibody production ability (half hemolytic value method), and immune organ index (spleen index and thymus index) in mice.
【学位授予单位】：中国海洋大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R392

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