小鼠胚泡围着床期LongSAGE文库构建及相关基因研究

发布时间：2018-01-08 13:26

本文关键词：小鼠胚泡围着床期LongSAGE文库构建及相关基因研究　出处：《重庆医科大学》2006年博士论文　论文类型：学位论文

【摘要】： 胚胎着床是人和哺乳动物生殖过程中妊娠建立的第一步,是决定妊娠成功与否的关键。成功的植入取决于处于接受态的子宫内膜和具有侵入性的胚胎间的同步协调反应。在植入开始,母体与胚胎建立了密切的物质和信息交流。目前认为有多种分子和细胞水平的调控分子如内分泌、旁分泌、邻分泌调节因子参与胚胎的着床过程,并通过多种细胞信号途径从时间和空间上对胚胎和子宫进行精细调控。但是子宫内膜仅在某一特定时期允许胚胎着床,这一时期时间很短,代表着子宫内膜对胚胎具有容受性,称为“着床窗口期”。胚胎着床过程包括脱去透明带、定位、黏附、侵入到最后着床等几个阶段。由于涉及到众多基因的调控以及错综复杂的调节因子网络的参与,早期的胚胎发育和着床的详细分子机制尚不清楚。几乎所有生物学变化都与关键基因的表达有关。人们以往一直试图从某一特定的分子与基因作为突破口,探寻着床发生机制。现在大规模的基因表达谱分析已广泛应用于生物学和医学研究领域,其中寡核苷酸芯片、DNA微阵列以及基因表达系列分析是目前应用最广泛的基因表达分析方法。但DNA微阵列只能检测已知基因,且定量分析受到限制,而SAGE技术能较完整地获得基因组表达丰度的数量信息,能够大规模对转录本进行定性和定量分析,可充分了解基因转录组的全貌,甚至可以发现新基因,被证明是一种对全基因组基因进行表达分析和寻找差异表达基因的强有力工具。通过对转录本特定位置的短寡核苷酸标签(即SAGE标签)的鉴定、分析可以获取基因表达的信息。
[Abstract]:Embryo implantation is the first step of the reproductive process and the establishment of pregnancy in mammals, is key to successful pregnancy. Successful implantation depends on the endometrium is in the receiving state and has invaded synchronous reactive embryos. In maternal and embryo implantation, established the close material and information exchange. At present there are many molecules that control the molecular and cellular levels such as endocrine, paracrine and juxtacrine regulator in embryo implantation process, and through a variety of cellular signaling pathways from time and space of the embryo and the uterus for fine regulation. But the endometrium only in a specific period of time to allow embryo implantation during this period a short, representing the endometrial receptivity has on embryo, known as the "implantation window". The process of embryo implantation includes taking off transparent, positioning, adhesion, invasion to the end with bed etc. The molecular mechanism of early embryo development and implantation is not clear due to the regulation of many genes and the involvement of complex regulatory network.
The expression of almost all biological changes and key genes. People in the past has been trying as a breakthrough from a particular gene and molecular mechanism of implantation. Now, explore the large-scale gene expression profile analysis has been widely applied in the fields of biology and medical research, the oligonucleotide microarray, DNA microarray and serial analysis of gene expression is expressed at present the most widely used gene analysis method. But the DNA microarray can detect known genes, and quantitative analysis is limited, and SAGE technology can better obtain the expression of the number of genomic information abundance, can be large-scale on the transcription of the qualitative and quantitative analysis, can fully understand the gene transcription group picture, can even find new genes, is that is a powerful tool for analyzing and identifying differentially expressed genes on the whole genome. Based on this. Specific location of the short oligonucleotide label (SAGE tag) identification, analysis can obtain the information of gene expression.

【学位授予单位】：重庆医科大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R321.3

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