胚胎期大鼠端脑和脊髓的发育及TrkA的表达

发布时间：2018-01-08 17:15

本文关键词：胚胎期大鼠端脑和脊髓的发育及TrkA的表达　出处：《吉林大学》2006年硕士论文　论文类型：学位论文

【摘要】：本实验研究了胚胎期Wistar大鼠端脑及脊髓发育的基本过程。结果显示E13天胎鼠神经管已闭合,形成前端脑泡。此期前端脑泡尚未分化成端脑(见附图1),脑泡上皮仍处于神经管上皮阶段;脊髓横断面呈实质块状,神经细胞排列密集,嗜碱性较强,未见到神经纤维形成。至E15天,端脑开始发育,古皮质形成,纹状体原基形成;此期的脊髓横断面呈栗子状,背侧神经细胞发育较快,HE染色嗜碱性较强,仍未见到神经纤维形成。E17天始,新皮质形成;脊髓灰质横断面已渐呈蝴蝶结状,背侧双角神经元发育较前角快,细胞较小,HE染色嗜碱性较强;中央管横断面呈菱形;神经纤维已清晰可见;在前角内可见散在的尼氏体阳性细胞,胞体较大,多呈菱形。E19至E21天,端脑及脊髓的轮廓均已呈成年时形状,端脑皮质细胞分层渐清晰,脊髓前角内神经元清晰可见,神经元胞体较大。同时本实验还观察了神经元的发育情况。尼氏体于E15天始检测到,但数量极少;E17天后数量逐渐增加,直至E19天。E13天至E15天(少量)虽未检测到尼氏体,但此期的细胞小而圆,排列密集,嗜碱性强,说明此期是神经元或神经前体细胞的大量增殖期,而E19天后,至E21天,未见到神经元数量的明显增加,但胞体增大明显,这说明此期是神经元成熟期。本实验在观察了大鼠胚胎时期端脑及脊髓的发育过程的基础上,利用常规免疫组织化学方法探讨了神经生长因子特异性受体TrkA在胚胎发育不同时期端脑及脊髓内的表达情况。结果发现E13天时胎鼠端脑及脊髓内均未见到TrkA阳性表达,此时Nestin染色呈弱阳性,说明此阶段处于神经管发育的后期,还未形成特征性
[Abstract]:The basic process of the development of the terminal brain and spinal cord in embryonic Wistar rats was studied. The results showed that the neural tube of embryonic rat was closed on day 13. The anterior vesicles had not been differentiated into telencephalon at this stage (see Fig. 1), and the vesicular epithelium was still in the neurotubule epithelium stage. The transverse section of the spinal cord was parenchymal block, the nerve cells were dense and basophilic, and no nerve fibers were formed. On the 15th day of E15, the telencephalon began to develop, the paleocortex formed and the striatum primordium formed. The spinal cord in this stage was chestnut shaped, and the dorsal nerve cells developed faster and stronger basophilic with HE staining. However, the formation of nerve fibers and neocortex was not seen on the 17th day after the formation of nerve fibers. The cross section of the gray matter of the spinal cord had gradually appeared as a bow knot, the dorsal biangular neurons developed faster than the anterior horn, and the cells were smaller and stronger basophilic in HE staining. The cross section of the central tube was rhomboid. Nerve fibers were clearly visible. Scattered Nissl body positive cells were found in the anterior horn. The cell bodies were larger, most of them were rhombic. E19 to E21 days. The contours of the terminal brain and spinal cord were in the shape of adulthood, and the stratification of the terminal cortical cells was gradually clear. The neurons in the anterior horn of the spinal cord were clearly visible and the neuronal bodies were large. The development of neurons was also observed in this experiment. The Nissl's body was detected at the 15th day of E15, but the number was very small. After 17 days of E17, the number of cells increased gradually, until days E19, E13 to E15 (a little), although no Nieldite bodies were detected, the cells in this phase were small and round, densely arranged and strongly basophilic. The results showed that the proliferation of neurons or neural precursor cells was significant in this period, but no significant increase in the number of neurons was observed after E19 days and at 21 days after E21, but the increase of cell body was obvious. This indicates that this period is the mature stage of neurons. This experiment was based on the observation of the development of cerebrum and spinal cord in embryonic rats. The expression of nerve growth factor specific receptor (TrkA) in the telencephalon and spinal cord of fetal rats at different stages of embryonic development was studied by routine immunohistochemical method. The results showed that there was no expression of NGF-specific receptor TrkA in the telencephalon and spinal cord of fetal rats at day 13. Positive expression of TrkA was observed. At this time, Nestin staining was weakly positive, indicating that this stage was in the late stage of neural tube development and had not yet formed a characteristic.
【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R321

【参考文献】