A型肉毒毒素对电刺激神经引发的骨骼肌单收缩和强直性收缩的作用模式

发布时间：2018-01-09 00:28

本文关键词：A型肉毒毒素对电刺激神经引发的骨骼肌单收缩和强直性收缩的作用模式　出处：《兰州大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：已知A型肉毒毒素(BTX-A)作用于骨骼肌神经肌肉接点突触前膜，切割神经突触相关蛋白SNAP-25，，使胞吐现象不能发生，突触囊储存的乙酰胆碱不能释放。该项目是研究BTX-A对电刺激坐骨神经引发蟾蜍腓肠肌单收缩及强直收缩的作用，分析不同剂量的BTX-A在不同作用时间所产生的肌收缩张力的变化曲线，旨在探究BTX-A对骨骼肌作用模式，为进一步寻找能阻止神经芽的旁生和突触前膜功能恢复的药物试验提供动物模型。方法：将蟾蜍(体重80～90g)随机分为2组。(1)琥珀胆碱组(SCh，一种能与骨骼肌神经肌肉接点突触后膜上的N_2胆碱受体结合并激动受体，使突触后膜对递质乙酰胆碱不产生反应的药物)：SCh组进一步分为药物注射组和对照组，每只蟾蜍右侧腓肠肌注射SCh 0.04mg(n=44)，左侧腓肠肌注射0.2ml任氏液(RS)作为对照(n=44)。(2)BTX-A组：BTX-A组进一步分为药物注射组和对照组，每只蟾蜍右侧腓肠肌分别注射BTX-A0.75U(n=48)、1.25U(n=42)、2.5U(n=42)和5.0U(n=42)，左侧腓肠肌注射0.2ml RS作为对照(n=134)(3)将注射过BTX-A、SCh和RS的蟾蜍分别于1、4、7、10、24和48小时制备成坐骨神经腓肠肌标本。单发电刺激(0.5 Hz，2V)和连续电刺激(20Hz，2V)坐骨神经引发腓肠肌等长收缩，通过肌肉张力换能器将该收缩力变化进行图像显示和记
[Abstract]:Objective: it is known that botulinum toxin type A (BTX-A) acts on the neuromuscular junctional presynaptic membrane of skeletal muscle and cleans the neurosynaptic associated protein SNAP-25 so that exocytosis can not occur. Acetylcholine stored in synaptic sac can not be released. This project is to study the effects of BTX-A on the electrical stimulation of sciatic nerve to induce the contraction of toad gastrocnemius muscle. The change curve of muscle contractile tension caused by different doses of BTX-A at different time was analyzed in order to explore the mode of action of BTX-A on skeletal muscle. Animal models were provided for further drug experiments to prevent paracentesis and presynaptic membrane function recovery of nerve buds. Methods: toad (body weight 80g / 90g) was randomly divided into two groups: succinylcholine group (SCh). The present invention relates to a NST-2 choline receptor that binds to the neuromuscular junction of skeletal muscle and excites the receptor on the postsynaptic membrane. The postsynaptic membrane did not react to acetylcholine in the postsynaptic membrane. The control group was further divided into two groups: the drug injection group and the control group. Each toad was injected with SCh 0.04 mg / L in the right gastrocnemius. The left gastrocnemius muscle was injected with 0.2ml of Ren's liquid RSs) as control group (n = 44) BTX-A group was further divided into two groups: drug injection group and control group. Each toad's right gastrocnemius muscle was injected with BTX-A 0.75 U ~ (25) U ~ (2 +) and 5.0 U ~ (5) U ~ (n) ~ (42) respectively. Left gastrocnemius muscle was injected with 0.2ml RS as control group. The gastrocnemius muscle specimens were prepared at 24 and 48 hours. The isometric contraction of gastrocnemius muscle was induced by single electrical stimulation (0.5 Hz) and continuous electrical stimulation (20 Hz ~ 2V). The change of the contraction force is displayed and recorded by the muscle tension transducer.
【学位授予单位】：兰州大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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