维生素C预处理对脱氧雪腐镰刀菌烯醇诱导的免疫抑制作用影响的实验研究

发布时间：2018-01-09 07:17

本文关键词：维生素C预处理对脱氧雪腐镰刀菌烯醇诱导的免疫抑制作用影响的实验研究　出处：《河北医科大学》2006年硕士论文　论文类型：学位论文

【摘要】：目的：单端孢霉烯族毒素是一组结构类似的真菌代谢产物，包括某些已知的最有效的蛋白合成抑制因子，可导致动物和人类的许多中毒症状。脱氧雪腐镰刀菌烯醇(deoxynivalenol，DON)又名呕吐毒素(vomitoxin VT)，为雪腐镰刀菌烯醇的脱氧衍生物，主要由真菌属镰刀菌产生。雨量过多，，气候寒冷以及不恰当的储藏条件都可能导致镰刀菌滋生并产生大量呕吐毒素，因此在全世界范围内，DON污染谷类粮食及其制品的情况非常普遍。研究表明，我国食管癌高发区居民的饮食中DON是主要污染真菌毒素之一。动物实验结果提示，体重增加下降，拒绝进食和呕吐是DON的慢性毒性作用，而摄入低剂量的DON能够损伤宿主的体液免疫和细胞免疫功能，从而降低机体的抵抗力。鉴于DON对人类及动物免疫系统产生的危害，如何降低和消除DON的这一负面作用受到学者的广泛关注。流行病学调查表明微量元素、维生素缺乏及营养失衡与区域性高发病—食管癌密切相关，而营养干预措施已被广泛应用于食管癌高发区的二级预防。本研究结合我国食管癌高发区真菌毒素污染和营养素缺乏现状，分别采用细胞培养、流式细胞定量检测技术、
[Abstract]:Objective: Monosporin is a group of similar fungal metabolites, including some of the most effective protein synthesis inhibitors known. Deoxynivalenolol deoxynivalenol (DON), also known as vomitoxin VT, can cause many toxic symptoms in animals and humans. A deoxyderivative of nivalenol, mainly produced by Fusarium fungi. Excessive rainfall, cold weather, and inappropriate storage conditions can cause Fusarium to breed and produce large amounts of vomit toxins. Therefore, it is very common to pollute cereals and their products around the world. DON is one of the major contaminated mycotoxins in the diet of residents with high incidence of esophageal cancer in China. The results of animal experiments indicate that weight loss, refusal to eat and vomiting are the chronic toxic effects of DON. Intake of low doses of DON can damage the host's humoral and cellular immune function, thus reducing the body's resistance. In view of the harm of DON to human and animal immune system. How to reduce and eliminate this negative role of DON has been widely concerned by scholars. Epidemiological investigation showed that trace elements, vitamin deficiency and nutritional imbalance were closely related to the regional high incidence of esophageal cancer, and nutrition intervention has been widely used in secondary prevention of esophageal cancer in high incidence areas. According to the current situation of mycotoxin contamination and nutrient deficiency in high incidence areas of esophageal cancer in China, cell culture and flow cytometry were used in this study.
【学位授予单位】：河北医科大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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