家猪骨髓基质细胞的多向分化与透明质酸成骨作用的实验研究

发布时间：2018-01-10 07:25

本文关键词：家猪骨髓基质细胞的多向分化与透明质酸成骨作用的实验研究　出处：《南昌大学》2006年博士论文　论文类型：学位论文

【摘要】：长期以来，自体骨移植一直是骨的修复与重建方法的金标准。由于自体骨移植存在许多不足，，如取材有限、失血、延长手术时间、疤痕形成、增加病人痛苦等，因此，寻找安全有效的替代自体骨移植的方法成为世界性研究热点。近年来，随着材料科学的进展，骨组织工程技术得到了迅速发展。然而，基于支架材料的骨组织工程技术所遇到的一些基本问题尚未完全解决，阻碍了该技术在临床上的广泛应用。这些基本问题包括：种子细胞的来源、种子细胞的扩增与分化调控、以及支架材料的选择等问题。骨髓基质细胞(BMSC)是骨髓中的一类非造血干细胞，它不仅具有支持和调节骨髓造血的功能，而且在适宜的条件下可向成骨细胞、软骨细胞和脂肪细胞等分化，并且能在体外自我更新，成为理想的组织工程种子细胞。迄今为止，已从人、大鼠、小鼠、鸡、牛、狗、猪等骨髓中分离出BMSC。自从Friedenstein等(1976)首次利用贴塑料壁的特点从成体骨髓中分离出具有成骨潜能的BMSC以来，人们对其分化特性及其机制进行了大量的研究。BMSC分化调控通路受多种细胞因子、激素、物理作用等复杂因素的影响。这些因素在转录因子水平通过调控各自分化的特异性转录因子，从而最终决定细胞分化方向。骨髓基质细胞成骨分化的一般特点是成骨细胞形态的形成、碱性磷酸酶(ALP)活性的增加以及包含Ⅰ型胶原(Coll)、骨涎蛋白(BSP)、骨连接素(ON)和骨钙素(OC)等骨基质蛋白的骨样矿化形成。核心结合因子a1(cbfa1)和位于cbfa1的下游的osterix被认为是骨髓基质细胞成骨分化的最关键转录因
[Abstract]:For a long time, autologous bone transplantation has been the gold standard of bone repair and reconstruction. There are many shortcomings in autogenous bone transplantation, such as limited materials, blood loss, prolonged operation time, scar formation, increased pain of patients and so on. Therefore, finding a safe and effective alternative to autogenous bone transplantation has become a worldwide research hotspot. In recent years, with the development of material science, bone tissue engineering technology has been rapidly developed. Some of the basic problems encountered in bone tissue engineering based on scaffold materials have not been completely resolved, which hinders the wide application of the technology in clinical practice. These basic problems include the source of seed cells. The regulation of seed cell proliferation and differentiation, as well as the selection of scaffold materials, etc. Bone marrow stromal cells (BMSCs) are a kind of non-hematopoietic stem cells in bone marrow. They not only support and regulate the hematopoiesis of bone marrow, but also can be transferred to osteoblasts under suitable conditions. Chondrocytes and adipocytes are differentiated and can renew themselves in vitro and become ideal seed cells for tissue engineering. So far, it has been from humans, rats, mice, chickens, cattle, dogs. BMSCs were isolated from porcine bone marrow. BMSC with osteogenic potential has been isolated from adult bone marrow for the first time since Friedenstein et al. 1976. Many studies have been carried out on its differentiation characteristics and its mechanism. BMSC differentiation regulation pathway is subject to a variety of cytokines and hormones. The effects of complex factors, such as physical effects, on transcription factors at the level of transcription factors by regulating the specific transcription factors of their differentiation. The general characteristics of osteogenic differentiation of bone marrow stromal cells are the formation of osteoblasts, the increase of alkaline phosphatase (ALP) activity and the inclusion of type I collagen. Bone sialoprotein (BSP). Bone like mineralization of bone matrix proteins such as osteonectin (ON) and osteocalcin (OC). Core binding factor a1 / cbfa1). Osterix, located downstream of cbfa1, is considered to be the most critical transcriptional cause of osteogenic differentiation in bone marrow stromal cells
【学位授予单位】：南昌大学
【学位级别】：博士
【学位授予年份】：2006
【分类号】：R329.2

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