噪声对孕鼠T、B淋巴细胞及胎鼠肺SP-B mRNA的影响

发布时间：2018-01-10 17:28

本文关键词：噪声对孕鼠T、B淋巴细胞及胎鼠肺SP-B mRNA的影响　出处：《吉林大学》2006年硕士论文　论文类型：学位论文

【摘要】：随着现代化生活的进步,噪声污染已经日益严重。为研究噪声对母体免疫系统及子代的影响,本实验以孕鼠为实验对象构建噪声污染模型,运用RIA法测孕鼠血清皮质酮水平,MTT法测孕鼠T、B淋巴细胞转化功能,RT-PCR法测胎肺SP-B mRNA的表达水平。结果如下:随着噪声强度的增加、作用时间的延长,实验组孕鼠的血清皮质酮水平逐渐增加;孕鼠体重增加值逐渐下降;孕鼠胸腺指数、脾脏指数逐渐下降;孕鼠T、B淋巴细胞的刺激指数逐渐下降;胎鼠体重逐渐下降;胎肺表达的SP-B mRNA水平逐渐下降。结论:大鼠在孕期接受噪声刺激后,机体产生的皮质酮水平升高,其免疫系统功能受到抑制;且可以导致胎儿宫内发育迟缓,IUGR肺发育不成熟。孕期噪声暴露对母儿产生上述不良影响,其影响程度与噪声呈强度-效应、时间-效应关系,与机体产生的皮质酮呈浓度-效应关系。
[Abstract]:With the progress of modern life, noise pollution has become increasingly serious. In order to study the effect of noise on the maternal immune system and offspring of pregnant rats in this experiment for noise pollution model experiment object construction, measuring the serum corticosterone level in pregnant rats by RIA method, T MTT method of measuring pregnant rats, B lymphocyte transformation function, expression SP-B mRNA measurement of fetal lung RT-PCR method. The results are as follows: with the increase of the noise strength, the prolongation of time, the serum corticosterone level in pregnant rats in experiment group increased gradually; the weight of pregnant rats increased gradually decreased; pregnant rat thymus index, spleen index decreased gradually; pregnant rats T, B lymphocyte stimulation index decreased gradually; the fetal body weight decreased gradually; fetal lung expression of SP-B mRNA decreased. Conclusion: the rats received noise stimulation during pregnancy, the body produces elevated corticosterone levels, the immune system is inhibited and can cause fetal; Intrauterine growth retardation and IUGR lung development are not mature. During pregnancy, noise exposure has adverse effects on mothers and children. The degree of influence is related to noise intensity, time effect relationship and corticosterone effect.

【学位授予单位】：吉林大学
【学位级别】：硕士
【学位授予年份】：2006
【分类号】：R363

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